DIAGNOSTIC RADIOLOGY-IMAGING-METABOLISM OF TUMOR

我们动物研究的总体目标是评估不同的 体外功能和形态参数与MRI/31P-MMR的比较 以及脑肿瘤生长和对类固醇治疗反应的PET数据。宠物 数据将包括血流量、氧气消耗量和 18F-2-氟-2-脱氧葡萄糖摄取。这些研究旨在 使用体内MRI/MRS和PET技术以及 建立了体外检测程序。在犬类工作中，MRI和PET 研究将在相同的动物身上进行，使用本地化 将提供相同组织样本检查的技术 音量。这两种非侵入性活体手术的结果将是 与体外详细的形态计量学-组织学分析相关 肿瘤以及脑/肿瘤组织的比重测量和 使用微球技术测定血流量。高分辨率 (显微镜)成像程序将用于研究兔脑 肿瘤作为其生长和治疗反应的函数 减少肿瘤引起的浮肿。核磁共振的高分辨率图像将是 对照样本体积面积的详细组织学评价。 肿瘤相关的水肿将通过测量TL和 T2弛豫时间及其与体外直接测量肿瘤的比较 水肿性脑组织水含量及血浆蛋白渗出 以及血管-血管外间隙测定。这个 拟议的研究及时解决了与 MRI/MRS提供的信息和潜在的临床应用 还有PET。

The overall goal of our animal studies is to evaluate how various in vitro functional and morphological parameters compare with MRI/31p-MMR and PET data of brain tumor growth and response to steroid therapy. PET data will include blood flow, oxygen consumptionand 18F-2-Fluoro-2-deoxyglucose uptake. The studies are designed to quantitate data using in vivo MRI/MRS and PET techniques as well as established in vitro assay procedures. In the canine work, MRI and PET studies will be performed on the same animals using localization techniques that will provide examination of the same tissue sample volume. The results of both non invasive in vivo procedures will be correlated to in vitro detailed morphometric-histological analysis of the tumors as well as brain/ tumor tissue specific gravity measurements and blood flow determinations using microsphere techniques. High resolution (microscopy) imaging procedures will be employed to study rabbit brain tumors as a function of their growth and response to therapy aimed at reducing tumor induced edema. The MRI high resolution images will be compared to detailed histological evaluation of the sample volume area. Tumor associated edema will be quantitated using measurements of Tl and T2 relaxation times and compared to direct in vitro measurements of tumor and edematous brain tissue water content extravasation of plasma proteins and vascular - extravascular space compartment determinations. The proposed studies address timely and important issues relating to the information provided and the potential clinical applications of MRI/MRS and PET.