13 ERA IB: ANTHOcyanin production PLatform Using Suspension cultures (ANTHOPLUS)

花青素是一种水溶性色素，可以给许多植物的果实和花朵着色。已鉴定出635种不同的花色苷，通过甲基化、糖基化和与脂肪族和芳香族基团的酰化来区分。越来越多的证据表明，食用富含花青素的食物有助于健康，最近对蓝莓、越橘和小红莓等富含花青素的水果的研究也支持了这一点。花色苷在饮食中的相对丰富，以及对一系列慢性病的效力，使其成为实验和预防医学以及最近用于配制天然色素的密集研究的主题。然而，商业上可获得的花青素的有限范围和纯制剂的费用意味着大多数研究都是使用植物的粗提物进行的，这些粗提物没有关于它们所含的特定花青素的标准化，也没有对提取物中每个花青素的量进行标准化。花色苷装饰的不同导致了花色苷在颜色稳定性和色调上的差异，并强调了开发纯花色苷生产系统的必要性，以研究化学专一性对医学应用的花色苷吸收、信号和生理、毒性的影响，以及开发天然色素行业的新配方。在ANTHOPLUS中，将开发新的植物细胞培养物，以稳定生产各种花色苷。产生这些文化的新颖程序意味着它们不受现有技术的限制。独一无二的是，我们的细胞培养可以持续、高水平地生产各种花青素。增加纯花色苷的供应将对着色剂行业研究装饰物、共色素、pH对颜色和稳定性的影响具有重要价值，为开发天然着色剂的新植物来源和天然色素的新配方提供坚实的科学基础。

Anthocyanins are water-soluble pigments that colour the fruit and flowers of many plants. More than 635 different anthocyanins have been identified, distinguished by methylation, glycosylation and acylation with both aliphatic and aromatic groups. There is mounting evidence that consumption of anthocyanin-rich food promotes health, supported by many recent studies of anthocyanin-rich fruits such as blueberry, bilberry and cranberry. Their relative abundance in the diet and their potency against a range of chronic diseases have made anthocyanins the subject of intense research in experimental and preventive medicine and, more recently for formulating natural colours. However, the limited range of anthocyanins commercially available and the expense of pure preparations mean that most research is done with crude extracts of plants which are not standardised with respect to the particular anthocyanins they contain, nor the amounts of each anthocyanin in the extract. Variations in anthocyanin decoration account for differences in colour stability and hue of anthocyanins and underpin the need for developing production systems for pure anthocyanins for investigating the effects of chemical specificity on uptake, signalling and physiology, toxicity of anthocyanins for medical applications and for developing new formulations in the natural colours industries. In ANTHOPLUS novel plant cell cultures will be developed for the stable production of a wide variety of anthocyanins. The novel procedure for generating these cultures means that they are not constrained by prior art. Our cell cultures, uniquely, allow sustained, high level production of diverse anthocyanins. Enhanced supplies of pure anthocyanins will be highly valuable for the colourants industry to investigate the effects of decorations, co-pigments, pH on colour and stability to provide a robust scientific foundation for developing new plant sources of natural colourants and new formulations for natural colours.