BIOSYNTHESIS OF THE ANTITUMOR ALKALOIDS OF CATHARANTHUS

吲哚生物碱生物合成的生物合成图谱 将使用无细胞培养来研究长春花属植物 从改良的组织培养物和植物匀浆中提取。这个 色氨酸和色氨酸的重排和转化 番木素直到复杂的二聚体溶瘤生物碱 将检查长春花碱和长春新碱。其作用机制 体内操作将应用于经济地合成一种 广泛的二聚体生物碱，到目前为止只有少量 生物活性评估的金额，使用合并的 部分合成和酶转化策略。 对玫瑰花细胞的基因操作将允许新的生物碱 与世隔绝以及增加产量的机会 转化植株中的VLb和VCR。 植物新陈代谢的高场核磁共振研究将得到发展。

Biosynthetic mapping of indole alkaloid biosynthesis in Catharanthus roseus will be studied using cell-free incubations from modified tissue cultures and plant homogenates. The rearrangements and transformations from tryptophan and secologanin up to the complex dimeric oncolytic alkaloids vinblastine and vincristine will be examined. The mechanisms operating in vivo will be applied to the economical synthesis of a wide range of dimeric alkaloids, hitherto only available in small amounts for biological activity evaluation, using the combined strategies of partial synthesis and enzymatic transformation. Genetic manipulation of C. roseus cells will allow novel alkaloids to be isolated as well as the opportunity to increase the yield of VLB and VCR in transformed plants. High-field NMR studies of plant metabolism will be developed.