BBSRC-Embrapa Genomics and pathogenomics to improve FHB and brusone resistance in Brazilian wheat

项目团队项目团队将研究领导者与巴西和英国小麦宿主和FHB和布鲁森真菌病原体的互补专业知识相结合。Pedro Scheeren（Embrapa）和Eduardo Caierao（Embrapa）拥有无与伦比的巴西小麦生产知识，提供小麦育种专业知识以及种质抗性状态知识，这对于进行全基因组关联扫描以确定与FHB和布鲁松抗性相关的基因组区域至关重要。Cristobal Uauy（JIC）因其在小麦遗传学和谷物基因克隆方面的专业知识而获得国际认可。CU与Ksenia Krasileva（TGAC/TSL）密切合作，后者领导小麦功能基因组学工具的开发，包括外显子组捕获平台的开发，这将成为该项目的支柱之一。Paul Nicholson（JIC）领导的研究小组调查了谷物对FHB抗性的遗传基础，并将与Flávio桑塔纳和Maria Imaculada Moreira Lima（Embrapa）合作，为该项目中的宿主和病原体成分做出贡献。James Cockram（NIAB）在将分子遗传学和基因组学与统计技术相结合以研究小麦的复杂性状方面拥有丰富的经验。这些技能将与领导数量遗传学计划的Luciano Consoli（Embrapa）合作使用，以进行巴西小麦种质中目标性状的关联映射。James Cockram将为Embrapa人员提供培训，使他们能够在获得FHB和brusone数据时进行额外的分析。Joao Leodato（Embrapa）是brusone疾病方面的公认专家，将与Gisele托雷斯（Embrapa）一起工作，他在使用功能基因组学分析处理Brusone病原体和与小麦宿主相互作用方面拥有丰富的经验。Antonio Nhani Jr（Embrapa）利用基因组学研究布鲁森病原体的毒力和宿主特异性。Embrapa研究人员提供的技能和知识将与尼克塔尔博特（埃克塞特大学）的技能和知识相辅相成，后者在布鲁森真菌的遗传学和毒力方面的专业知识得到国际认可。Diane Saunders（TGAC/JIC）领导了病原体种群和毒力的研究，为FHB和布鲁松病原体的种群结构提供了重要的见解，这些结构将用于宿主抗性遗传学的研究。除了Embrapa和英国参与者的高度互补的技能组合外，这些组织还提供互补的资源和基础设施。Embrapa在巴西各地的环境最有利于FHB和布鲁森病的地区拥有出色的田间试验设施和训练有素的人员，使他们能够进行高质量的疾病试验。位于帕索丰多的Embrapa工厂配备了实验室、计算基础设施、温室和植物生长设施，其环境能够使植物快速生长，从而最大限度地缩短世代时间，并确保植物种群有效发育至基因分型和表型分型所需的固定水平。该项目的英国合作伙伴（JIC、TGAC、NIAB和埃克塞特）都拥有广泛的实验室、计算和植物生长设施，这是该项目的宿主和病原体遗传学和基因组学组成部分所需的。此外，TGAC拥有最先进的基因组测序和转录分析设施以及计算机硬件和软件，以进行相关的数据处理和分析。

Project teamThe project team combines research leaders with complementary expertise in both the wheat host and the FHB and brusone fungal pathogens in Brazil and the UK. Pedro Scheeren (Embrapa) and Eduardo Caierao (Embrapa) have unrivalled knowledge of Brazilian wheat production, providing expertise in wheat breeding alongside knowledge of germplasm resistance status that is essential for genome wide association scans to be undertaken to identify genomic regions associated with resistance to FHB and brusone. Cristobal Uauy (JIC) is internationally recognised for his expertise in wheat genetics and gene cloning in cereals. CU collaborates closely with Ksenia Krasileva (TGAC/TSL) who leads the development of functional genomics tools in wheat, including the development of exome-capture platforms that will form one of the pillars of the project. Paul Nicholson (JIC) leads a research group investigating the genetic basis of resistance to FHB in cereals and will contribute to both host and pathogen components in the project in partnership with Flávio Santana and Maria Imaculada Moreira Lima (Embrapa), who lead research on FHB resistance. James Cockram (NIAB) has extensive experience in combining molecular genetics and genomics with statistical techniques to investigate complex traits in wheat. These skills will be used in collaboration with Luciano Consoli (Embrapa) who leads the quantitative genetics programme, to undertake association mapping of target traits in Brazilian wheat germplasm. James Cockram will provide training to Embrapa personnel to enable them to undertake additional analyses on FHB and brusone data as these become available.Joao Leodato (Embrapa) is a recognised expert on brusone disease and will work alongside Gisele Torres (Embrapa) who has extensive experience of working with the Brusone pathogen and interactions with the wheat host using functional genomics analysis. Antonio Nhani Jr (Embrapa) employs genomics to investigate virulence and host specificity in the brusone pathogen. The skills and knowledge provided by Embrapa researchers will be complemented those of Nick Talbot (University of Exeter) who is internationally recognised for his expertise in the genetics and virulence of the brusone fungus. Diane Saunders (TGAC/JIC) leads research into pathogen populations and virulence, providing essential insight into the structure of the populations of the FHB and brusone pathogens that will be utilised within studies of the genetics of host resistance. Resources and InfrastructureIn addition to the highly complementary skill sets of the Embrapa and UK participants, the organisations contribute complementary resources and infrastructure. Embrapa has excellent field trials facilities and trained personnel across Brazil in regions where the environment is most conducive to the FHB and brusone diseases enabling them to undertake high quality disease trials. The Embrapa site at Passo Fundo is exceptionally well equipped with laboratory, computing infrastructure, glasshouse and plant growth facilities in an environment that enables the rapid growth of plants to minimise generation time and ensure efficient development of plant populations to the required level of fixation for genotyping and phenotyping purposes. The UK partners within this project (JIC, TGAC, NIAB and Exeter) all have extensive laboratory, computational and plant growth facilities, required for the host and pathogen genetics and genomics components of the project. In addition, TGAC has state of the art genome sequencing and transcriptional profiling facilities and computer hardware and software to undertake the associated data processing and analysis.