BIOCHEMISTRY OF SALIVARY LIPIDS

该项目前几年进行的研究提供了 唾液腺分泌物中脂质的详细表征， 结石 拟议调查的长期目标是： 以确定唾液脂质与蛋白质的关系， 唾液中的糖蛋白成分在健康和疾病;并建立 唾液脂质在膜形成过程中的作用。 的 具体目标是：确定脂质的含量和组成 与低分子量和高分子量粘蛋白型糖蛋白相关 从下颌下-舌下唾液中纯化;以建立结构 甘油糖脂与低浓度和高浓度 分子量粘蛋白，并确定这种相互作用是否涉及 粘蛋白的蛋白质或碳水化合物成分;确定 脂质对唾液粘蛋白流变学性质的影响;分离和 表征腮腺唾液中的脂蛋白;研究 唾液腺中甘油糖脂的生物合成; 在薄膜形成过程中沉积在牙齿表面的脂质 并建立甘油糖脂的要求，在其他方面 唾液脂质和/或蛋白质，用于它们在釉质表面上的吸附; 为了提高我们对人体内脂质成分和水平的认识， 在健康和疾病中的主要和次要唾液腺的分泌物。 低分子量和高分子量粘液糖蛋白将从 防龋和易感龋的下颌下-舌下唾液 通过凝胶过滤的两阶段程序， CsCl密度梯度平衡离心。 腮腺分离 抗龋和易龋受试者的唾液 通过NaBr密度梯度将用于分离 脂蛋白 大鼠腮腺和下颌下腺将用于 甘油糖脂生物合成的体外研究。 脂质 将分析在体内和体外形成的薄膜的组分。 体外成膜系统将用于研究 甘油糖脂在牙釉质上吸附的需要量 表面。 主要和次要分泌物中脂质的数据 患有全身性疾病和唾液腺疾病的患者的唾液腺将 与健康人相比。

The studies conducted during the previous years of this project provided detailed characterization of lipids in salivary gland secretions and concretions. The long-term objectives of the proposed investigations are: to define the relationship of salivary lipids to the protein and glycoprotein components of saliva in health and disease; and to establish the role of salivary lipids in the process of pellicle formation. The specific goals are: to determine the content and composition of lipids associated with the low and high molecular weight mucin-type glycoproteins purified from submandibular-sublingual saliva; to establish the structural requirements of glyceroglucolipids for interacting with the low and high molecular weight mucins, and to determine whether this interaction involves the protein or carbohydrate component of mucins; to determine the effect of lipids on the rheological properties of salivary mucins; to isolate and characterize the lipoproteins from parotid saliva; to study the biosynthesis of glyceroglucolipids in salivary glands; to characterize the lipids deposited on the tooth surfaces in the process of pellicle formation and to establish the requirements of glyceroglucolipids, in terms of other salivary lipids and/or proteins, for their adsorption on enamel surfaces; to advance our knowledge on the composition and levels of lipids in the secretions of the major and minor salivary glands in health and disease. The low and high molecular weight mucus glycoproteins will be isolated from submandibular-sublingual saliva of caries-resistant and caries-susceptible individuals by a two stage procedure of gel filtration followed by equilibrium centrifugation in CsCl density gradient. Separation of parotid saliva from caries-resistant and caries-susceptible subjects by flotation through NaBr density gradient will be employed for isolation of lipoproteins. Rat parotid and submandibular glands will be utilized in the in vitro studies on biosynthesis of glyceroglucolipids. The lipid components of the pellicle formed in vivo and in vitro will be analyzed. The in vitro pellicle formation system will be used to study the requirements of glyceroglucolipids for their adsorption on enamel surfaces. The data on lipids in the secretion of the major and minor salivary glands of patients with systemic and salivary gland diseases will be compared with those of healthy individuals.