REGULATION OF INTESTINAL ION TRANSPORT

目标是进一步界定与监管有关的因素。 上皮细胞离子转运。具体地说，网站(S)和机制由 肠道离子转运的内源性调节因子有哪些变化 家兔降结肠对电解质的吸收和分泌 将对体外实验进行研究。跨上皮离子通量的测量， 将进行细胞内电位和离子活性的研究。这些 技术的设计是为了进一步定义：(1)假定的影响 肠离子跨上皮转运的内源性介质 钠、钾和氯在结肠中的转运；(2)这些介质的作用 关于细胞内膜电位、膜电阻和 细胞内离子活性；(3)这些介质的作用部位(S)； 以及(4)钙调素和前列腺素在刺激分泌中的作用 在肠子里偶联。结果应提供以下信息 内源性调节因子对肠道离子转运的影响 吸收刺激和分泌刺激的作用部位和机制。是这样的 信息对于允许科学合理的治疗是必不可少的 中医辨证论治盐运失调症的探讨 肠子。此外，由于各种不同的运输特性 吸收上皮和分泌上皮相似，对 存在于该上皮细胞的吸收和分泌机制可能为 关于肠道病理生理学的重要信息 特别是运输过程和吸收和吸收的生理学。 一般为分泌性上皮。

The objectives are to further define the factors involved in regulation of epithelial ion transport. Specifically, the site(s) and mechanisms by which putative endogenous regulators of intestinal ion transport alter absorption and secretion of electrolytes by the rabbit descending colon in vitro will be investigated. Measurements of transepithelial ion fluxes, intracellular potentials and ion activities will be conducted. These techniques are designed to further define: (1) the effects of putative endogenous mediators of intestinal ion transport on the transepithelial transport of Na, K and Cl in the colon; (2) the effects of these mediators on intracellular membrane potentials, membrane resistances and intracellular ion activites; (3) the site(s) of action of these mediators; and (4) the role of calmodulin and prostaglandins in stimulus-secretion coupling in the intestine. Results should provide information concerning the effects of endogenous regulators on intestinal ion transport and the sites and mechanisms by which absorptive and secretory stimuli act. Such information is essential to allow scientifically rational therapeutic approaches to the treatment of disorders of salt and water transport by the intestine. Furthermore, since the transport characteristics of a variety of absorptive and secretory epithelial are similar, studies of the absorptive and secretory mechanisms present in this epithelia may provide important information concerning the pathophysiology of intestinal transport processes in particular and the physiology of absorptive and secretory epithelia in general.