PANACEA: Pathogenomics for enhancing food security in East Africa

大豆是全球约3亿消费者的营养素、维生素和矿物质的重要来源。虫害和疾病，如灰假尾孢菌引起的角斑病，可严重降低产量和质量，导致营养不良和收入损失。农民通常依赖于种植抗性品种来对抗疾病的影响，但不幸的是ALS是遗传多样性的，因此选择表现最好的品种可能是有问题的。为ALS等真菌病原体的病理分型提供资源可以帮助研究人员和植物育种家改善疾病管理，品种选择并指导新的抗性品种的开发，以帮助种植者稳定生产力并降低疾病爆发的发生率和严重程度。该项目将有助于建立新的、创新的生物学和生物信息学资源，为当地专家和研究人员提供更高的能力，以准确监测ALS种族的差异。这将使新的或新颖的病理类型能够在出现时被识别，并且如果它们对增长区域构成潜在威胁，则允许及时进行干预。这些资源将包括：i）从乌干达9个不同的农业生态系统中取样的75-100个灰霉病菌分离株的初始病原体收集ii）围绕小组开发的基因组资源，为病理分型和功能研究提供基因分型信息iii）使用新的基因组资源的种群多样性筛选，以开始阐明影响东非豆类作物的ALS病理型iv）系列会议，网络研讨会和研讨会，以优化新的资源，用户实用程序，并向利益相关者和当地专家演示新资源。新资源将开始用于促进改善对菜豆上ALS病原体灰斑病菌的监测和监视，以帮助预防未来疾病爆发，指导品种选择和新品种培育。这将直接有助于稳定产量和生产力，提高小农户和数百万依赖普通豆类作为廉价营养食品来源的消费者的营养摄入量和收入。这些资源也将更广泛地应用于对病原体基因组学和流行病学更基本方面感兴趣的科学研究人员。项目期间制定的初步框架可适用于其他作物/病原体，以支持进一步的改进工作。

SummaryCommon bean is an essential source of nutrients, vitamins and minerals to approximately 300 million consumers worldwide. Pest and disease, such as angular leaf spot (ALS) caused by Pseudocercospora griseola, can seriously reduce yields and quality, leading to malnutrition and lost incomes. Farmers often rely on growing resistant varieties to combat the effects of disease but unfortunately ALS is genetically diverse, so selecting the best-performing varieties can be problematic. Providing resources for pathotyping fungal pathogens like ALS can help researchers and plant breeders to improve disease management, variety selection and guide the development of new, resistant varieties to help growers to stabilise productivity and to reduce the incidence and severity of disease outbreaks. The pump-priming project will help to establish new, innovative biological and bioinformatics resources that will provide local experts and researchers with enhanced capability to accurately monitor for differences in ALS races. This will enable new or novel pathotypes to be identified when arise, and if they pose a potential threat to a growing region, allowing for interventions to be made in good time. The resources will comprise of:i) An initial pathogen collection of 75-100 P. griseola isolates sampled from across the nine different Ugandan agro-ecologiesii) Genomic resources developed around the panel providing genotyping information for pathotyping and functional studiesiii) A population diversity screen using the new genomic resource to begin to shed light on ALS pathotypes affecting East African bean cropsiv) Series of meetings, webinars and workshops to optimise the new resources for end-user utility and demonstrate the new resource to stakeholders and local experts.The new resources will begin to facilitate improved monitoring and surveillance of P. griseola the causal agent of ALS on common bean in order to help prevent future disease outbreaks and to guide variety selection and the breeding of new varieties. This will contribute directly to stabilise yields and productivity, enhancing nutritional intakes and incomes for small-holder farmers and the millions of consumers dependent on common bean as a cheap, nutritious source of food. The resources will also have wider application to scientific researchers with interests in more fundamental aspects of pathogen genomics and epidemiology. The initial framework developed during the project could be applied to other crops/pathogens to support further improvement efforts.