18-BTT EAGER: A system for the production of haploid inducer lines and cytoplasmic male sterile doubled haploids for efficient hybrid production

杂交品种是现代作物生产的基石。种子公司花了很多年的时间来开发优良的近交亲本系，用于生产农民每个季节种植的杂交种子。植物育种周期中的一个主要瓶颈是需要反复回交有希望的亲本系以使它们完全近交。一个节省时间的解决方案是使用双单倍体在一代中直接产生完全近交系。通过这种方法，首先分离出单倍体植物，然后使用化学处理使其染色体加倍。由此产生的双单倍体根据定义是完全近交的。为了使该策略对植物育种者具有实际用途，需要用于产生初始单倍体植物的可靠方法。在这个提议中，我们的目标是开发和测试一个通用的策略，使单倍体诱导系，应该提供植物育种工具，他们需要有效地产生双单倍体杂交种子生产的精英亲本系。

Hybrid cultivars are a cornerstone of modern crop production. Seed companies spend many years developing elite inbred parental lines that are used to produce the hybrid seed that farmers plant each season. A major bottleneck in the plant breeding cycle is the need to repeatedly backcross promising parental lines to make them fully inbred. One time-saving solution to this challenge is to use double haploids to directly produce fully inbred lines in one generation. With this approach a haploid plant is first isolated, and then chemical treatment is used to double its chromosomes. The resulting double haploid is by definition fully inbred. For this strategy to be of practical use to plant breeders, a reliable method for producing the initial haploid plants is required. In this proposal our objective is to develop and test a general strategy for making haploid inducer lines that should provide plant breeders with the tool they need to efficiently produce double haploid elite parental lines for hybrid seed production.