STRUCTURE AND FUNCTION OF NUCLEIC ACIDS

这个项目的目标是对 核酸的构象和支配它们的力的构象。我们 将在核酸的碱基序列之间寻找有用的关联 以及其结构和生物功能。更好地理解 核酸可能有助于预防或治愈以下所有疾病 依赖于核酸的功能。这些疾病包括病毒性疾病， 遗传病和癌症。 将合成特定碱基序列的寡核苷酸以提供 DNA、RNA和DNA-RNA杂交物的结构元素。热力学和 错配互补双链螺旋形成的动力学 螺旋线和发夹环将被测量。构象将是 用一维和二维核磁在溶液中确定 共振测量。 核酸中的三级和更高阶折叠结构将是 研究了圆偏振光的吸收和散射。

The goal of this project is to obtain a fundamental understanding of the conformations of nucleic acids and of the forces which govern them. We will seek useful correlations between the base sequence of a nucleic acid and its structure and biological function. A better understanding of nucleic acids may help in the prevention or cure of all diseases which depend on the functions of nucleic acids. These include viral diseases, genetic diseases and cancer. Oligonucleotides of specific base sequence will be synthesized to provide structural elements of DNA, RNA and DNA-RNA hybrids. Thermodynamics and kinetics of formation of complementary double-strand helices, mismatched helices and hairpin loops will be measured. Conformations will be determined in solution using one-and two- dimensional nuclear magnetic resonance measurements. Tertiary and higher-order folded structures in nucleic acids will be studied using absorption and scattering of circularly polarized light.