'Horticulture' CRISPR Cas-mediated and inter-species transfer of broad-spectrum, potentially durable disease resistance in crop plants (CRIMIST-DR).

这种病毒被称为芜菁花叶病毒（TuMV），是一种重要的病原体，感染许多作物类型，降低产量，使它们无法销售。为了繁殖，病毒必须利用植物中的某些蛋白质。没有这些蛋白质，它就不能繁殖。一种这样的植物蛋白被称为真核起始因子iso4E [eIF（iso）4E]。我们已经证明，在大白菜（白菜亚种），其中有三个副本的eIF（iso）4E，如果其中一个副本丢失，病毒不能复制，但植物不受影响。目前，一家国际种子公司（先正达）正在开发具有这种抗性的大白菜品种（在沃里克大学鉴定和鉴定）。大白菜是世界上最重要的蔬菜作物。我们一直无法在相关和重要的植物物种甘蓝中鉴定出对TuMV的任何抗性，这些植物物种包括西兰花、卷心菜、花椰菜、羽衣甘蓝、布鲁塞尔芽甘蓝等。在伊丽莎白克里克园艺技术中心和沃里克大学植物病毒学小组、NIAB和商业植物育种公司先正达的合作下，我们的目标是敲除TuMV在B中复制所需的eIF（iso）4E的拷贝。甘蓝利用基因编辑技术，从而建立了一种快速开发不同B的病毒抗性品种的技术。在与商业种子公司先正达的合作中，我们还旨在将该病毒不能从大白菜中使用的eIF（iso）4E拷贝转移到B中。通过传统的杂交方法培育出抗病毒的植物。

The virus called turnip mosaic virus (TuMV) is an important pathogen infecting many crop plant types, reducing yields and making them unmarketable. In order to reproduce, the virus has to use certain proteins in plants. Without these proteins it cannot reproduce. One such plant protein is called the eukaryotic initiation factor iso4E [eIF(iso)4E]. We have shown that in Chinese cabbage (Brassica rapa sub-species pekinensis) which has three copies of eIF(iso)4E, if one of the copies is missing, the virus cannot reproduce, but the plant is unaffected. Chinese cabbage varieties with this resistance (that was identified and characterised at the University of Warwick) are currently being developed by an international seed company (Syngenta). Chinese cabbage is the most important vegetable brassica crop worldwide.We have been unable to identify any resistance to TuMV in the related and important plant species Brassica oleracea, which includes broccoli, cabbage, cauliflower, kale, Brussels sprouts etc. In collaboration between the Elizabeth Creak Horticultural Technology Centre and the Plant Virology Group at the University of Warwick, NIAB and the commercial plant breeding company Syngenta, we aim to knock out the copy of eIF(iso)4E that TuMV needs in order to reproduce in B. oleracea utilising gene editing technology, thereby establishing a technique to rapidly develop virus-resistant varieties of the different B. oleracea types.In collaboration with the commercial seed company Syngenta, we also aim to move the copy of eIF(iso)4E that the virus cannot use from Chinese cabbage into B. oleracea by conventional crossing, in order to develop virus-resistant plants by this alternative route.