STRUCTURE OF DENITRIFICATION ENZYMES

拟议的项目将确定酶的结构要素 在异化反硝化中负责亚硝酸盐还原。 质子 核磁共振光谱将用于确定结构 关于催化位点血红素基团，包括表征 电子国。 共振拉曼光谱将用于 研究催化位点的振动状态。 的确切 酶的辅基的化学结构将是 通过从完整的酶中纯化这些酶和结构确定， 通过核磁共振、振动、光学和 质谱法 氮氧化物与整个细胞的相互作用 也将被审查。 目标酶系统是酶系统的代表。 电子传递酶和蛋白质的一般类别。 其研究将 促进对这门课的基本理解， 负责所有细胞的能量产生和呼吸。

The proposed project will determine structural elements for the enzyme responsible for nitrite reduction in dissimilatory denitrification. Proton nuclear magnetic resonance spectroscopy will be used to determine structure about the catalytic site heme groups, including the characterization of electronic states. Resonance raman spectroscopy will be used to investigate the vibrational states of the catalytic sites. The exact chemical structure of the prosthetic groups of the enzyme will be determined by purification of these from the intact enzyme and a structural determination through nuclear magnetic resonance, vibrational, optical, and mass spectroscopy. The interactions of oxides of nitrogen with whole cells will also be examined. The target enzyme system is representative of the general class of electron transport enzymes and proteins. Its study will promote the understanding on a fundamental level of this class which is responsible for energy production and respiration in all cells.