ROLE OF INTEGRATIVE HOST FACTOR IN GENE EXPRESSION

大肠大肠杆菌组蛋白样蛋白，IHF，直接作用于允许 Lambda基因的翻译，cII。 我们的假设是， 这是通过改变cII转录本的结构， 接近核糖体; IHF结合位点位于邻近cII 夏恩-达尔加诺序列 我们希望通过以下方式来分析这一监管体系： 遗传和生物化学方法。 前者是我们的便利。 许多cII基因和操纵子融合的构建;后者由我们的 建立了一个转录-翻译偶联系统， cII表达的IHF要求。 我们还计划探索 cII mRNA的结构。 最后，我们提供选择协议，以隔离 cII表达不依赖于IHF的宿主突变体;这些突变体可以 显示由IHF突变引起的其他表型的改变。

An E. coli histone-like protein, IHF, acts directly to allow the translation of a Lambda gene, cII. Our hypothesis is that it accomplishes this by altering the structure of the cII transcript so that it becomes accessible to ribosomes; an IHF binding site lies adjacent to the cII Shine-Dalgarno sequence. We wish to analyze this regulatory system by genetic and biochemical approaches. The former is facilitated by our construction of numerous cII gene and operon fusions; the latter by our establishment of coupled transcription-translation system which reflects the IHF requirement for cII expression. We plan also to probe the structure of cII mRNA. Finally, we offer selection protocols to isolate host mutants in which cII expression is IHF-independent; these mutants may display alterations in the other phenotypes caused by an IHF-mutation.