STRUCTURE OF SULFGLOBINS

硫化血红蛋白和硫化肌红蛋白是两种罕见的无功能 在血液和组织中发现的天然球蛋白的形式。他们 也可以在受控条件下在体外形成。过去时 研究表明，硫球蛋白是由 一些含硫部分与亚铁血红素的共价加成 天然珠蛋白的假体基团。这个项目将 确定这些不寻常衍生品的结构。这个 经修饰的血红素将被分离和稳定，通过选择 提取条件或通过转化为更稳定的 保留原始修改记录的派生项。 分离的亚铁血红素将以质子核为特征 磁共振、光学、红外光谱和质量 光谱分析来确定其结构。并行的 实验中，全蛋白的溶液结构 磺胺肌胶将用质子核磁法测定 共鸣。

Sulfhemoglobin and sulfmyoglobin are two unusual nonfunctional forms of the native globins found in blood and tissue. They may also be formed in vitro under controlled conditions. Past research has shown that the sulfglobins are produced by the covalent addition of some sulfur containing moiety to the heme prosthetic group of the native globins. This project will determine the structure of these unusual derivatives. The modified heme will be isolated and stabilized by choice of extraction conditions or by conversion to a more stable derivative that retains a record of the original modification. The isolated heme will be characterized by proton nuclear magnetic resonance, optical, infra-red spectroscopy, and mass spectrometry to determine its structure. In parallel experiments, the solution structure of the whole protein sulfmyoglovin will be determined by proton nuclear magnetic resonance.