GERM CELL MIGRATION IN DROSOPHILA

这项研究的长期目标是了解细胞组是如何 在胚胎的不同位置相互作用形成一个 结构复杂。这份提案概述了一项关于发展和 一个这样的结构的组装，果蝇的胚胎性腺 果蝇。了解性腺是如何组装的需要 了解更多关于细胞规范、识别、 运动性和细胞间的交流。细胞的相似过程 专门化和迁徙是脊椎动物和 处于正常发育和疾病中的无脊椎动物系统，如 在转移性肿瘤的侵袭和扩散方面。 这项建议有三个具体目标： (I)确定果蝇nudC基因在生殖细胞中的作用 运动和性腺组装。果蝇nudC是一个新的基因，它是 在生殖细胞中表达，并可能在核或细胞中发挥作用 能动性。该基因是用来自一名患者的抗血清鉴定的。 自身免疫性疾病系统性红斑狼疮。该基因编码一种 与细胞核运动所需的蛋白质50%同源的蛋白质 真菌曲霉，nudC基因。 (2)确定极电池移动所需的位置线索，并 胚胎性腺形成。性腺的组装可以被分离 分成四个独立的步骤，每个步骤都由不同的线索调节。 这一特定目标寻求确定所需的位置线索 生殖细胞运动和胚胎性腺的组装。 (Iii)确定与性腺组装有关的基因 通过收集不足的股票，第三个具体目标是寻求 发现新的合子基因，这些基因在生殖细胞运动和 性腺组件。这个特定的目标勾勒出合子基因的筛查。 使用覆盖约三分之二的删除股票的集合 基因组。

The long term objective of this research is to learn how groups of cells at different locations in an embryo interact with each other to form a complex structure. This proposal outlines a study of the development and assembly of one such structure, the embryonic gonad of the fruitfly Drosophila . Understanding how the gonad is assembled will require learning more about the mechanism of cell specification, recognition, motility and cell-cell communication. Similar processes of cell specification and migration are characteristic of vertebrate and invertebrate systems both in normal development and in disease such as in the invasiveness and spread of metastatic tumors. This proposal has three specific aims: (I) To determine the role the Drosophila nudC gene plays in germ cell movement and gonad assembly. Drosophila nudC is a novel gene which is expressed in germ cells and is likely to play a role in nuclear or cell motility. The gene was identified using antisera from a patient with the autoimmune disease systemic lupus erythematosus. The gene encodes a protein 50% homologous to a protein required for nuclear movement in the fungus Aspergillus, the nudC gene. (II) To identify the positional cues necessary for pole cell movement and embryonic gonad formation. The assembly of the gonad can be separated into four separate steps each of which is mediated by distinct cues. This specific aim seeks to determine the positional cues required for germ cell movement and the assembly of the embryonic gonad. (III) To identify genes that are involved in the assembly of the gonad by collection of deficiency stocks, The third specific aim seeks to identify novel zygotic genes which play a role in germ cell movement and gonad assembly. This specific aim outlines a screen for zygotic genes using a collection of deletion stocks that cover about two thirds of the genome.