HIGH MASS MOLECULAR IONS BY ELECTROHYDRODYNAMIC EMISSION

目标是开发用于质量分析的仪器， 蛋白质和其他生物大分子的范围为10，000至 10万AMU I期研究的具体目的是证明 离子源产生蛋白质分子离子的可行性 其根据电流体动力学（EHD）发射原理操作。 这 交替电离方法被提出作为"软"电离方法- 减少了由碎片离子种类引入的光谱背景。 甘油溶液，而不是更挥发性的溶剂，将被探讨 用于高质量排放研究。 甘油的优点是低蒸汽 压力，允许施加更高的电场之前， 分解的开始和低温下的高粘度。 两 条件是非常有利的，以提高排放量 大分子离子 大分子离子发射特性将 在溶剂流动、离子强度和样品方面进行研究 浓度. 预计带电液滴抑制和 优选的离子发射将通过控制溶剂 通过冷却EHD发射器来提高产量和粘度。 TOF测量将 用于测量离子束的颗粒尺寸分布。

The objective is to develop instrumentation for the mass analysis of proteins and other biological macromolecules in the range of 10,000 to 100,000 AMU. The specific aim of Phase I studies is to demonstrate the feasibility of producing protein molecular ions generated by ion sources which operate on the principle of electrohydrodynamic (EHD) emission. This alternate ionization method is proposed as a "soft" ionization method - reducing the background in spectra introduced by fragment ion species. Glycerol solutions, as opposed to more volatile solvents, will be explored for high mass emission studies. The advantage of glycerol are low vapor pressure, permitting the application of higher electric fields before the onset of breakdown, and high viscosity at low temperatures. Both conditions are highly favorable for enhancing the emission of macromolecular ions. The macromolecular ion emission characteristics will be investigated with respect to solvent flow, ionic strength and sample concentration. It is anticipated that charged droplet suppression and preferred ion emission will be demonstrated by controlling the solvent throughput and viscosity by cooling the EHD emitter. TOF measurements will be used to measure the particle size distribution of the ion beam.