DISPERSION OF DIFFUSIBLE INDICATORS IN THE BODY

该计划的重点是小溶质的运输机制 从血液进入心肌， 通过反式-和 内皮细胞间途径。 以前的工作已经走了很长的路， 获得血管内和 经毛细血管转运 新的重点是对机制， 转运，特别是脂肪酸和腺苷进入或穿过 内皮细胞通过易化或被动机制。 多 将使用指示剂稀释技术来评价单向 腺苷和脂肪酸的质膜通量。 具体实验 将做测试的假设，膜转运蛋白为 腺苷和肌苷可以作为促进反向转运蛋白， 是，显示增加顺式到反式通量，通过提高 反浓度。 该计划应导致证明新技术 用于估计完整器官中穿过内皮细胞壁的通量， 提供了犬和兔心肌中特定溶质的数据。

The proposed program is focused on mechanisms of transport of small solutes from the blood into the myocardium and in particular on the characterization of transport across the capillary wall, via trans- and inter-endothelial cell routes. Previous work has gone a long way in obtaining clear mathematical descriptions of intravascular and transcapillary transport. The new emphasis is on the mechanisms of transport, particularly for fatty acids and adenosine into or across endothelial cells via facilitated or passive mechanisms. The multiple indicator dilution technique will be used to evaluate the unidirectional plasmalemmal fluxes of adenosine and fatty acids. Specific experiments will be done to test the hypothesis that the membrane transporters for adenosine and inosine can behave as facilitating countertransporters, that is, show augmentation of cis to trans flux by raising the trans-concentrations. The program should result in proven new techniques for estimating fluxes across endothelial cell walls in intact organs and provide data on specific solutes in the myocardium of dogs and rabbits.