PATHOGEN-FREE BREEDING AND RESEARCH

猴T淋巴细胞嗜性病毒I型 免疫缺陷病毒和D型逆转录病毒是重要的动物模型 研究人类免疫缺陷，也构成了威胁， 圈养家养繁殖群体的健康状况。 猴疱疹病毒是一种 已证明对动物护理人员和实验室人员有危害。 新 英格兰地区灵长类动物研究中心（NERPRC）已成功 建立了无特定病原体（SPF）恒河猴繁殖群 以提供未受感染的实验对象并改善群体健康。 我们建议把殖民地的数目增至250只，以支持这些建议。 关键目标。 繁殖地将自给自足， 每年35只SPF恒河猴， 在NERPRC的研究项目。 未感染的动物将被识别 使用成熟的血清学和病毒分离技术。 SPF动物将 将被集体安置在一个专用的现代混凝土砌块建筑中。 的SPF 将通过定期血清学检查确保殖民地的状态 监视 繁殖群体的遗传多样性 通过跟踪个体动物谱系以避免近亲繁殖来最大化 并通过分析定期测定平均杂合性 血液蛋白多态性 行为管理和研究将确保 SPF育种者具有正常的社会和母性特征。

The simian retroviruses simian T-lymphotropic virus I, simian immunodeficiency virus, and type D retrovirus are important animal models for the study of human immunodeficiency and also pose a threat to the health of captive domestic breeding colonies. Herpesvirus simiae is a proven hazard to animal care workers and laboratory personnel. New England Regional Primate Research Center (NERPRC) has successfully established a specific-pathogen-free (SPF) rhesus monkey breeding colony to provide uninfected experimental subjects and to improve colony health. We propose to expand the colony to 250 monkeys in support of these crucial goals. The breeding colony will be self-sustaining and provide 35 SPF rhesus monkeys annually for the ongoing and Federally funded research programs at the NERPRC. Uninfected animals will be identified using proven serologic and virus isolation techniques. SPF animals will be group housed in a dedicated modern concrete block building. The SPF status of the colony will be ensured through periodic serologic surveillance. The genetic diversity of the breeding colony will be maximized by tracking of individual animal pedigrees to avoid inbreeding and by periodic determination of average heterozygosity through analysis blood protein polymorphism. Behavioral management and study will ensure that SPF breeders have normal social and maternal characteristics.