MOLECULAR GENETIC ANALYSIS OF LYMPHOCYTE FUNCTION

这些研究的重点是MHC的功能作用 I类分子及其与T细胞受体的相互作用。 模型 已经开发了用于在体内研究这些功能的系统， 体外 过去一年的成就包括：1）数量 测量纯化的T细胞受体与 纯化的MHC I类分子与一组抗原复合， 2）大量高纯度T细胞受体的工程化 结构域及其在结晶和功能研究中的应用; 3） 评估可溶性H-2Dd与携带H-Dd的NK细胞的相互作用。 Ly-49表面蛋白。 这些研究提供了对 MHC I类-肽复合物与 T淋巴细胞和NK细胞的受体。 它们也可能有助于产生 T细胞受体的详细结构 所有这些 调查将有助于开发一个更完整的分子图片， 涉及MHC I类分子的免疫识别事件有助于 调节这两种重要效应细胞类型的功能。

The focus of these studies has been on the functional role of the MHC class I molecule and its interactions with T cell receptors. Model systems have been developed for studies of these functions in vivo and in vitro. Accomplishments during the past year include: 1) quantitative measurement of the interactions between purified T cell receptors and purified MHC class I molecules complexed with a panel of antigenic peptides; 2) engineering of large amounts highly purified T cell receptor domains, and their use for crystallization and functional studies; 3) evaluation of the interaction of soluble H-2Dd with NK cells bearing the Ly-49 surface protein. These studies are providing a new quantitative understanding of the binding interactions between MHC class I-peptide complexes and the receptors of T lymphocytes and NK cells. They may also help produce a detailed structure of the T cell receptor. Together, these investigations will help develop a more complete molecular picture of how immune recognition events involving MHC class I molecules contribute to regulation of the functions of these two important effector cell types.