EXPRESSION, STRUCTURE AND FUNCTION OF LORICRIN, A MAJOR CELL ENVELOPE PROTEIN

皮质细胞被膜构成一个多组分的15 nm厚的 质膜内侧的一层高度不溶的蛋白质 终末分化的上皮细胞。 基于各种 数据，我们现在知道兜甲蛋白是表皮的主要成分， 细胞膜 它是通过N-（~-N-甲基丙烯酸甲酯）与包膜交联的。 谷氨酰）赖氨酸异二肽交联，由 转氨酶 兜甲蛋白是富含甘氨酸的蛋白质， 高度柔性甘氨酸环基序。 我们已经表达了人locricrin 在杆状病毒系统中研究其生化特性， 其交联的机制。 为了研究其精确的 在表皮中的作用，以及其表达的调节，我们已经使用了 转基因技术 一个含有全长人类基因的构建体 而侧翼序列可以指导适当的位点和阶段特异性 在转基因小鼠中过量表达人兜甲蛋白。 因为老鼠是 正常情况下，兜甲蛋白可能是一个非常晚的，如果不是最后，成分 添加到单元格信封中。

The cornified cell envelope constitutes a multi-component 15 nm thick layer of highly insoluble protein on the inside of the plasma membrane of terminally differentiated epithelial cells. Based on a variety of data, we now know that loricrin is a major component of the epidermal cell envelope. It is crosslinked to the envelope by way of N~-(~- glutamyl)lysine isodipeptide crosslinks, catalyzed by the action of transglutaminases. Loricrins are glycine-rich proteins that contain the highly flexible glycine loop motif. We have expressed human locricrin in a baculovirus system to study its biochemical properties and the mechanism(s) by which it is crosslinked. In order to study its precise role in the epidermis, and the regulation of its expression, we have used transgenic technology. A construct containing the full-length human gene and flanking sequences can direct the proper site- and stage-specific over-expression of human loricrin in transgenic mice. Since the mice are normal, loricrin is presumably a very late, if not the last, component to be added to the cell envelope.