CHARACTERIZATION OF A NOVEL HTLV-II CELL LINE

我们先前从一位再生障碍性贫血患者身上分离出一株细胞系(JR)。 产生HTLVII的贫血。我们已经确定了这一细胞系的特征 它产生的病毒。此外，从脐带衍生的第二个细胞系 感染JR病毒的血细胞。该细胞系仍为IL-2 依赖并且生长缓慢。两种细胞系的蛋白质印迹分析 显示了与其他HTLV-II病毒株相似的蛋白质图谱。 对使用IL-2受体的细胞系进行的Northern杂交分析显示 与其他感染细胞系相比，IL-2受体RNA水平较低。 我们正在研究使用JR-脐带血细胞系作为模型 用于早期HTLV感染。JR细胞系分离的研究 正在准备出版。

We previously isolated a cell line (JR) from a patient with aplastic anemia that produces HTLVII. We have characterized this cell line and the virus it produces. In addition, a second cell line derived from cord blood cells infected with the JR virus. This cell line remains IL-2 dependent and grows slowly. Western blot analysis of the two cell lines shows protein profiles similar to to other HTLV-II viral strains. Northern blot analysis of the cell lines used the IL-2 receptor, showed low levels of IL-2 receptor RNA compared to other infected cell lines. We are investigating the use of the JR-cord blood cell line as a model for early HTLV infection. Our studies on the isolation of JR cell line is being prepared for publication.