C-ETS GENE EXPRESSION DURING CELL PROLIFERATION AND DIFFERENTIATION

ets家族的基因编码转录因子并结合嘌呤- 丰富的序列存在于细胞的转录调控区， 和病毒启动子/增强子。 ets蛋白的DNA结合域 在家族的不同成员中是高度保守的。 同源性 在反式激活结构域区域保守性较低，表明 该家族的不同成员可能能够调节靶基因 不同的活动，取决于他们的具体互动， 其他转录因子。 为了了解 ets靶基因的调控，高度保守的DNA结合域 ETS 1及其突变形式在Jurkat T细胞中表达。 野生的- ETS 1型DNA结合结构域（ETS 1C）蛋白，但不是突变形式， 以更高的亲和力与ets结合DNA序列（EBS）结合， 比全长ETS 1蛋白解离更慢。 野生型ETS 1 C，而不是突变体，抑制EBS连接的氯霉素乙酰基 转移酶（CAT）报告基因的活性，并抑制生产 IL-2。 表达反义RNA以阻断ETS 1的形成 和ETS 2蛋白在淋巴细胞以及非淋巴细胞中的研究 它们在细胞增殖中的作用。

The ets family of genes encode transcription factors and bind to purine- rich sequences present in transcriptional regulatory regions of cellular and viral promoters/enhancers. The DNA binding domain of the ets protein is highly conserved among different members of the family. The homology is less conserved in the transactivation domain region, suggesting different members of the family may be able to modulate target gene activities differently, depending upon their specific interaction with other transcription factors. To understand the mechanisms involved in the regulation of ets targeted genes, highly conserved DNA binding domain of ETS1 and its mutant form are expressed in Jurkat T-cells. The wild- type ETS1 DNA binding domain (ETS1 C) protein, but not the mutant form, binds to ets binding DNA sequences (EBS) at higher affinity and dissociates more slowly than full-length ETS1 protein. Wild-type ETS1 C, but not mutant, inhibits the EBS linked chloramphenicol acetyl transferase (CAT) reporter gene activities and inhibits the production of IL-2. Antisense RNA is being expressed to block the formation of ETS1 and ETS2 proteins in lymphoid as well as non-lymphoid cells to study their role in cell proliferation.