REPRODUCTIVE BIOCHEMISTRY AND MEDICINE

本申请中提出的专业人口研究中心 目标是将假说和现代生物医学技术在 从分子生物学看生殖科学学科的连续体 生理学方面。原则、资源和专业知识的紧密结合 是最基本的。这项工作包括十个项目，分为两个部分 神经科学和早期发展的广泛范畴；I，关于 转化生长因子-β，II，哺乳动物精子发生的分析；III，关于 Hap26在果蝇中的表达，IV，促性腺激素释放激素脉冲的研究 灵长类动物中的生成器，V，生长的产前表达的研究 因子基因和受体；VI，恒河猴促性腺激素释放激素网络的研究 脑的神经解剖学方法；vii，胎盘CRH的分析 怀孕的狒狒，VIII，用类固醇/生长因子在 神经发育；IX，脑POMC和性激素调节的研究 黄体生成素和X，哺乳动物原癌基因表达分析 配子发生。组织了五个生物医学核心以加快技术 灵长类菌落的使用，分子细胞学/组织学技术， 类固醇和蛋白质、细胞和杂交的放射免疫分析 培养和分子生物学设施。

The Specialized Population Research Center proposed in this application targets a synthesis of hypotheses and modern biomedical techniques in a continuum of disciplines in reproductive sciences from molecular biology to physiology. Close integration of principles, resources and expertise is fundamental. The work includes ten projects which fall into the two broad categories of neuroscience and early development; I, a study of TGF-beta, II, an analyses of mammalian spermatogenesis; III, a study of hap26 expression in Drosophila, IV, the study of the GnRH pulse generator in primates, V, a study of the prenatal expression of growth factor genes and receptors; VI, a study of GnRH networks in the rhesus brain by neuroanatomical methods,; VII, analysis of placental CRH in pregnant baboons, VIII, experiments with steroid/growth factors in neurodevelopment; IX, study of sex steroid regulation of brain POMC and LH and X, an analysis of proto-oncogene expression during mammalian gametogenesis. Five biomedical cores are organized to expedite technical usage of a primate colony, molecular cytology/histology techniques, radioimmunoassay of steroids and proteins, cell and hybridization culture, and a molecular biology facility.