CHARACTERIZATION OF A NOVEL HTLV-II CELL LINE

我们以前分离的细胞系（JR）从患者再生障碍性贫血 产生HTLVII的细胞 我们已经鉴定了这个细胞系和病毒 它生产。 此外，第二种细胞系来源于脐带血细胞， 感染了JR病毒 该细胞系保持IL-2依赖性， 生长缓慢。 两种细胞系的蛋白质印迹分析显示， 与其他HTLV-II病毒株相似。 北方印迹分析 使用IL-2受体的细胞系显示低水平的IL-2受体， 与其他感染细胞系相比。 我们正在调查 JR-脐带血细胞系作为早期HTLV感染的模型。 我们对JR细胞系的分离的研究正在准备中， 出版物

We previously isolated a cell line (JR) from a patient with aplastic anemia that produces HTLVII. We have characterized this cell line and the virus it produces. In addition, a second cell line derived from cord blood cells infected with the JR virus. This cell line remains IL-2 dependent and grows slowly. Western blot analysis of the two cell lines shows protein profiles similar to other HTLV-II viral strains. Northern blot analysis of the cell lines used the IL-2 receptor, showed low levels of IL-2 receptor RNA compared to other infected cell lines. We are investigating the use of the JR-cord blood cell line as a model for early HTLV infection. Our studies on the isolation of JR cell line are being prepared for publication.