CULTIVATION OF MYCOBACTERIUM LEPRAE

麻风杆菌的体外增殖已经获得，并继续进行了 每隔30-60天进行9次传代。每毫升最好的数字 一直令人失望，稳定在每毫升1x10(6)-1x10(7)。它 可能是开发连续生长、大容量发酵罐所必需的 获取毫克或克量湿或干生长的系统。生长 使用佐剂也是为了提高生长速度。 随着细胞的收获。 分枝杆菌的一个新种，一种“犰螂衍生的分枝杆菌”(ADM)， 也已经成长起来。我们可以通过以下方法迅速将其与麻风杆菌区分开来 吡啶脱色，使麻风杆菌不耐酸。 ADM现在已经得到了充分的描述。根据生理学， 从遗传学和抗原性研究来看，它是一个新物种。最后的实验 麻风分枝杆菌的体外培养被推迟。斯科特·弗兰兹布洛 是这项研究的合作者之一。 他一直在为我们提供麻风杆菌细胞的最终测试 物种。我们可以从受感染的螳螂身上获取麻风菌的材料 (肝脏)。鼹鼠衍生的麻风杆菌总是有一种混合物 螳螂衍生的分枝杆菌，或ADM。大约有20种 这些，还没有被赋予名字。ADM在我们的房间里长满了麻风杆菌 养殖技术。斯科特·弗兰兹布劳的麻风菌感染裸鼠群体 最近被一场流行病消灭了。这些老鼠花了一年的时间 发展成感染。我们必须等这么久才能完成我们的实验。 进展：我们现在已经准备好，与Scott Franzblau合作， 完成生长实验。裸鼠麻风杆菌的素材是 再次从Franzblau博士处获得。利用这一点实现增长的问题 独特的方法将在几个月内决定。即使没有增长 证明，这项技术仍然有希望并将被发表 让其他人对它的延续感兴趣。

Multiplication of M. leprae has been obtained in vitro, and continued for nine passages at 30-60 day intervals. Best numbers per milliliter have been disappointing, leveling off at 1x10(6) - 1x10(7) per milliliter. It may be necessary to develop a continuous growth, large volume fermentor system to acquire milligram or gram amounts of wet or dry growth. Growth adjuvants are being used in an attempt to increase the growth rate as well as the cell harvest. A new species of mycobacterium, an "armadillo-derived mycobacterium" (ADM), has also grown out. We can quickly differentiate it from M. leprae by pyridine decolorization, which renders M. leprae, non-acid-fast. The ADM has now been fully characterized. According to physiological, genetic, and antigenic studies, it is a new species. Final experiments on the in vitro culture of Mycobacterium leprae are delayed. Scott Franzblau of the Hansen's disease center at Carville is a collaborator in this study. He has been supplying us with M. leprae cells definitive tests for the species. We have access to M. leprae material from infected armadillos (the liver). Armadillo-derived M. Leprae always has an admixture of armadillo-derived mycobacteria, or ADM. There are about twenty species of these, which have not been given names. The ADM overgrow M. leprae in our culture technique. Scott Franzblau's M. leprae infected nude mouse colony was recently wiped out with an epizootic. These mice take a year to develop infections. We must wait this long to finish out our experiment. Progress: We are ready now, in collaboration with Scott Franzblau, to complete the growth experiments. Nude mouse M. Leprae material is available once again from Dr. Franzblau. The question of growth using this unique method will be decided within a few months. Even if no growth is demonstrated, the technique would still have promise and would be published to interest others in its continuation.