BACTERIAL FUNCTION INVOLVED IN CELL GROWTH CONTROL

我们一直在研究蛋白质降解在调节 基因表达，并继续研究两者之间的联系 细胞分裂过程中染色体的合成和分裂。AS 关于荚膜多糖调控的部分后续研究 通过合成不稳定的调节子RCSA，我们发现了一个下游 RCSA合成的交易调节因子编码RNA，显然 调解监管效果。调节器和靶标的突变 将允许进一步描述监管模式的特征。突变 在RCSB的假定磷酸化位点支持 胶囊调节中的磷酸化。我们正在调查他所扮演的角色 热休克伴侣蛋白在Lon底物降解中的作用 ATP依赖的蛋白水解酶及其识别的初步研究 Lon的特异性。中电能源的角色及监管研究- 依赖于蛋白酶的菌株已经集中在为紧凑型 对CLP合成的调节，并研究一种新的功能 发现了另一种调节亚基ClpX。

We have been studying the role that protein degradation plays in regulating gene expression and have continued with studies on the linkages between chromosome synthesis and partition of chromosomes during cell division. As part of continuing studies on the regulation of capsular polysaccharide synthesis by the unstable regulator RcsA, we have found that a downstream transacting regulator of rcsA synthesis encodes RNAs which apparently mediate the regulatory effect. Mutations in both the regulator and target will allow further characterization of the mode of regulation. Mutations in the putative phosphorylation site of RcsB support a role for phosphorylation in capsule regulation. We are investigating the role of the heat shock chaperone proteins in degradation of substrates of the Lon ATP-dependent protease and initiating studies on the recognition specificity of Lon. Studies on the role and regulation of the Clp energy- dependent protease have concentrated on developing strains for the tight regulation of Clp synthesis, and investigating the function of a newly discovered alternate regulatory subunit, ClpX.