EFFECTS OF NEW ANTICANCER DRUGS ON THE CELL CYCLE

新型抗癌药物对细胞生长、细胞周期进程的影响 细胞活性和细胞分化将在以下方面进行研究：a)三 不同的细胞系(L1210小鼠白血病，Friend小鼠红白血病， 中国仓鼠卵巢(CH0)细胞；B)刺激的正常人淋巴细胞 C)多细胞肿瘤球体(V79细胞)。这个 选择不同的细胞模型来区分药物作用 从影响特定单元格类型的操作中通用，以比较 细胞周期对转化细胞与正常人细胞的影响 并确定实体肿瘤肿块中的生长如何影响 对构成细胞的药物作用。除了详细描述之外 研究表明，药物的作用机制将通过相关研究来探讨 上述观察对染色质结构、细胞 形态、RNA/DNA和蛋白质/DNA比值。因此，不平衡的程度 生长可以与细胞周期动力学效应相关，也可以与细胞周期动力学效应相关 染色质结构、有丝分裂纺锤体异常等 案例，以阐明药物作用的机制，它与 将研究天然和合成的核酸。流式细胞术， 必要时由放射自显影支持，将是治疗的主要方法 分析细胞周期效应。电子显微镜、生化和 如有需要，还将使用生物物理方法来研究 毒品行动。此外，我们还建议进一步发展流式细胞仪。 药物对细胞代谢影响的分析方法，以及 继续研究与调控机制相关的细胞内因子 可能成为药物作用靶点的细胞周期。拥有更好的 了解细胞周期、新的、更有效的药物和药物 可以设计组合，从而产生更有效的治疗 协议。

Effects of new anticancer drugs on cell growth, cell cycle progression, cell viability and cell differentiation will be investigated on: A) three different cell lines (L1210 murine leukemia, Friend murine erythroleukemia, Chinese hamster ovary (CH0) cells); B) normal human lymphocytes stimulated by mitogen; and C) multicellular tumor spheroid (V79 cells). The different cell models were chosen to distinguish drug actions that are universal from actions affecting a specific cell type, to compare the effect on transformed cells with normal human cells, of cycling with quiescent cells and to determine how growth in solid tumor masses affect drug action on the constituient cells. In addition to detailed descriptive studies, the mechanisms of drug action will be investigated by correlating the observations noted above with effects on chromatin structure, cell morphology, RNA/DNA and protein/DNA ratios. Thus, the degree of unbalanced growth can be correlated with cell cycle kinetic effects as can changes in chromatin structure, abnormalities of mitotic spindle etc. In selected cases, to elucidate the mechanism of drug action, its interactions with natural and synthetic nucleic acids will be studied. Flow cytomery, supported when necessary by autoradiography, will be the main method of analysing cell cycle effects. Electron microscopy, biochemical and biophysical methods will also be used when indicated to study mechanisms of drug action. In addition, we propose to further develop flow cytometric methods for the analysis of drug effects on cell metabolism, and to continue research on intracellular factors related to regulatory mechanisms of the cell cycle that may be targets for drug action. With a better understanding of the cell cycle, new, more effective drugs and drug combinations can be designed giving rise to more effective treatment protocols.