MONITOR UPTAKE, RETENTION, SUBCELL LOCALIZATION & LIFETIME OF FLUORESCENT DRUGS

测定了椭圆藤碱在活细胞中的摄取和结合。 使用Hoechst 33342共染色的人早幼粒细胞（HL-60）细胞， 双激光激发流式细胞仪系统。 相敏FCM 方法测定了椭圆藤碱的荧光寿命 结合在活的HL-60细胞内。 椭圆形荧光寿命 以时间和剂量依赖性方式改变，表明 椭圆藤碱与DNA、与其他细胞的不成比例的结合， 选民。 这项研究表明，测量摄取的效用 和化疗剂在活细胞群中的结合，和 证明了荧光寿命测量的有用性。 正在进行的荧光寿命研究旨在比较药物 在耐药HL-60细胞和野生型中的摄取和结合 细胞

The uptake and binding of ellipticine was determined in viable human promyelocytic (HL-60) cells co-stained with Hoechst 33342 using a two-laser excitation flow cytometry system. Phase-sensitive FCM methods were used to measure the fluorescence lifetime of ellipticine bound within viable HL-60 cells. Ellipticine fluorescence lifetime was altered in both a time and dose dependent manner, demonstrating the disproportionate binding of ellipticine to DNA, to other cellular constituents. This study showed the utility of measuring the uptake and binding of chemotherapeutic agents in viable cell populations, and demonstrates the usefulness of fluorescence lifetime measurements. Fluorescence lifetime studies in progress are designed to compare drug uptake and binding in drug-resistant HL-60 cells and the wild-type cells.