FLUORESCENT DRUG: MONITOR UPTAKE, RETENTION, SUBCELLULAR LOCALIZATION & LIFETIME

测定了椭圆藤碱在活细胞中的摄取和结合。 使用Hoechst 33342共染色的人早幼粒细胞（HL-60）细胞， 双激光激发流式细胞仪系统。 相敏FCM 方法测定了椭圆藤碱的荧光寿命 结合在活的HL-60细胞内。 椭圆形荧光寿命 以时间和剂量依赖性方式改变，表明 椭圆藤碱与DNA、与其他细胞的不成比例的结合， 选民。 这项研究表明，测量摄取的效用 和化疗剂在活细胞群中的结合，和 证明了荧光寿命测量的有用性。 相敏流式细胞术的进一步发展将提供 药物的分离和定量，包括阿霉素和其他 荧光药物，不同的亚细胞成分， 用常规方法解决。

The uptake and binding of ellipticine was determined in viable human promyelocytic (HL-60) cells co-stained with Hoechst 33342 using a two-laser excitation flow cytometry system. Phase-sensitive FCM methods were used to measure the fluorescence lifetime of ellipticine bound within viable HL-60 cells. Ellipticine fluorescence lifetime was altered in both a time and dose dependent manner, demonstrating the disproportionate binding of ellipticine to DNA, to other cellular constituents. This study showed the utility of measuring the uptake and binding of chemotherapeutic agents in viable cell populations, and demonstrates the usefulness of fluorescence lifetime measurements. Further development of phase-sensitive flow cytometry will provide for resolution and quantitation of drugs, including adriamycin and other fluorescent drugs, to different sub-cellular components which cannot be resolved by conventional methods.