FLUORESCENT DRUG: MONITOR UPTAKE, RETENTION, SUBCELLULAR LOCALIZATION & LIFETIME

荧光药物：监测摄取、保留、亚细胞定位

• 批准号: 6280910
• 负责人: ZBIGNIEW DARZYNKIEWICZ
• 金额: $ 0.71万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6280910
• 关键词: biomedical resource; human tissue; nucleic acids; proteins

The uptake and binding of ellipticine was determined in viable human promyelocytic (HL-60) cells co-stained with Hoechst 33342 using a two-laser excitation flow cytometry system. Phase-sensitive FCM methods were used to measure the fluorescence lifetime of ellipticine bound within viable HL-60 cells. Ellipticine fluorescence lifetime was altered in both a time and dose dependent manner, demonstrating the disproportionate binding of ellipticine to DNA, to other cellular constituents. This study showed the utility of measuring the uptake and binding of chemotherapeutic agents in viable cell populations, and demonstrates the usefulness of fluorescence lifetime measurements. Further development of phase-sensitive flow cytometry will provide for resolution and quantitation of drugs, including adriamycin and other fluorescent drugs, to different sub-cellular components which cannot be resolved by conventional methods.

在活体内测定椭圆碱的摄取和结合 人早幼粒细胞(HL-60)与Hoechst 33342共染色 双激光激发流式细胞术系统。相敏FCM 用多种方法测量了椭圆碱的荧光寿命 结合在存活的HL-60细胞内。椭圆素荧光寿命 以时间和剂量依赖的方式改变，展示了 椭圆碱与DNA或其他细胞的不成比例结合 选民。这项研究显示了测量摄取的效用 和活细胞群中化疗药物的结合，以及 展示了荧光寿命测量的用处。 相敏流式细胞术的进一步发展将为 药物的拆分和定量，包括阿霉素和其他 荧光药物，对不同的亚细胞成分不能 用常规方法解决。