MONITOR UPTAKE, RETENTION, SUBCELL LOCALIZATION & LIFETIME OF FLUORESCENT DRUGS

在活体内测定椭圆碱的摄取和结合 人早幼粒细胞(HL-60)与Hoechst 33342共染色 双激光激发流式细胞术系统。相敏FCM 用多种方法测量了椭圆碱的荧光寿命 结合在存活的HL-60细胞内。椭圆素荧光寿命 以时间和剂量依赖的方式改变，展示了 椭圆碱与DNA或其他细胞的不成比例结合 选民。这项研究显示了测量摄取的效用 和活细胞群中化疗药物的结合，以及 展示了荧光寿命测量的用处。 正在进行的荧光寿命研究旨在比较药物 耐药HL-60细胞和野生型细胞的摄取和结合 细胞。

The uptake and binding of ellipticine was determined in viable human promyelocytic (HL-60) cells co-stained with Hoechst 33342 using a two-laser excitation flow cytometry system. Phase-sensitive FCM methods were used to measure the fluorescence lifetime of ellipticine bound within viable HL-60 cells. Ellipticine fluorescence lifetime was altered in both a time and dose dependent manner, demonstrating the disproportionate binding of ellipticine to DNA, to other cellular constituents. This study showed the utility of measuring the uptake and binding of chemotherapeutic agents in viable cell populations, and demonstrates the usefulness of fluorescence lifetime measurements. Fluorescence lifetime studies in progress are designed to compare drug uptake and binding in drug-resistant HL-60 cells and the wild-type cells.