EXPRESSION OF CELL CYCLE DEPENDENT CYCLINS MEASURED BY FLOW CYTOMETRY

通过流式细胞术测量细胞周期依赖性细胞周期蛋白的表达

• 批准号: 6280914
• 负责人: ZBIGNIEW DARZYNKIEWICZ
• 金额: $ 0.36万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6280914
• 关键词: biomedical resource; human tissue; nucleic acids; proteins

The cyclins are a family of cell cycle-related proteins that are expressed at different times and are involved in regulation of cells through the various phases of the cell cycle. In general, cyclins D and E appear primarily during G1 phase, cyclin A during S and cyclin B in G2 phase. The cyclins complex with cyclin-dependent kinases and these complexes phosphorylate proteins that are important for traverse of cells through the cell cycle. Antibodies are now available to the major cyclins and utilization of these probes for analysis of cyclin expression provides an important tool for measuring the position and frequency of cells in the various phases of the cell cycle. Darzynkiewicz and co-workers have developed and utilized cell-labeling and FCM techniques to demonstrate the correlation of cyclin D and E expression and staurosporine-induced G1 arrest of human lymphocytes and the effects of G2 arrest and polyploidization of transformed cells and cyclin B. Utilizing similar method we are investigating the expression of cyclins on human diploid fibroblasts following exposure to alpha and gamma irradiation.

细胞周期蛋白是一个与细胞周期相关的蛋白家族，它们是 在不同的时间表达，参与细胞的调节 通过细胞周期的不同阶段。一般来说，细胞周期蛋白D E主要出现在G1期，Cyclin A出现在S时期，Cyclin B出现在G1期 处于G2期。细胞周期蛋白与细胞周期蛋白依赖性激酶的复合体和 这些复合体使对Traverse很重要的蛋白质磷酸化 细胞在整个细胞周期中的比例。现在，抗体可用于 主要细胞周期蛋白及其在细胞周期蛋白分析中的应用 Expression提供了测量位置的重要工具和 细胞在细胞周期的不同阶段的频率。 Darzynkiewicz和他的同事开发并利用了细胞标记 和FCM技术显示细胞周期蛋白D和E的相关性 星形孢菌素诱导人淋巴细胞G1期停滞及其表达 转化细胞G2期停滞和多倍化的影响 和细胞周期蛋白B。利用类似的方法，我们正在研究 细胞周期蛋白在人二倍体成纤维细胞中的表达 阿尔法和伽马辐射。