EXPRESSION OF CELL CYCLE DEPENDENT CYCLINS MEASURED BY FLOW CYTOMETRY

细胞周期蛋白是一个细胞周期相关蛋白家族， 在不同的时间表达，并参与细胞的调节 细胞周期的各个阶段。 一般来说，细胞周期蛋白D 和E主要出现在G1期，cyclin A出现在S期，cyclin B G2期。 细胞周期蛋白与细胞周期蛋白依赖性激酶的复合物， 这些复合物磷酸化蛋白质， 在细胞周期中的作用 抗体现在可用于 主要细胞周期蛋白和这些探针用于分析细胞周期蛋白的用途 表达式为测量位置提供了重要工具， 在细胞周期的各个阶段的细胞的频率。 Darzynkiewicz及其同事开发并利用了细胞标记技术， 流式细胞术技术证明细胞周期蛋白D和E的相关性 星形孢菌素诱导的人淋巴细胞G1期阻滞 以及G2期阻滞和转化细胞多倍化的影响 和细胞周期蛋白B。利用类似的方法，我们正在调查 暴露后人二倍体成纤维细胞周期蛋白的表达 α和γ射线的辐射。

The cyclins are a family of cell cycle-related proteins that are expressed at different times and are involved in regulation of cells through the various phases of the cell cycle. In general, cyclins D and E appear primarily during G1 phase, cyclin A during S and cyclin B in G2 phase. The cyclins complex with cyclin-dependent kinases and these complexes phosphorylate proteins that are important for traverse of cells through the cell cycle. Antibodies are now available to the major cyclins and utilization of these probes for analysis of cyclin expression provides an important tool for measuring the position and frequency of cells in the various phases of the cell cycle. Darzynkiewicz and co-workers have developed and utilized cell-labeling and FCM techniques to demonstrate the correlation of cyclin D and E expression and staurosporine-induced G1 arrest of human lymphocytes and the effects of G2 arrest and polyploidization of transformed cells and cyclin B. U tilizing similar method we are investigating the expression of cyclins on human diploid fibroblasts following exposure to alpha and gamma irradiation.