EXPRESSION OF CELL CYCLE DEPENDENT CYCLINS MEASURED BY FLOW CYTOMETRY

细胞周期蛋白是细胞周期相关蛋白家族 在不同时间表达并参与细胞调节 经历细胞周期的各个阶段。 一般来说，细胞周期蛋白 D 和 E 主要出现在 G1 期，细胞周期蛋白 A 主要出现在 S 期，细胞周期蛋白 B 处于G2期。 细胞周期蛋白与细胞周期蛋白依赖性激酶复合物 这些复合物磷酸化对穿越很重要的蛋白质 细胞通过细胞周期。 抗体现已上市 主要细胞周期蛋白以及利用这些探针分析细胞周期蛋白 表达式提供了一个重要的工具来测量位置和 细胞处于细胞周期各个阶段的频率。 Darzynkiewicz 和同事开发并利用了细胞标记 和 FCM 技术证明细胞周期蛋白 D 和 E 的相关性 人淋巴细胞的表达和十字孢菌素诱导的 G1 期阻滞 以及 G2 停滞和转化细胞多倍化的影响 和细胞周期蛋白 B。利用类似的方法，我们正在研究 暴露后人二倍体成纤维细胞上细胞周期蛋白的表达 α 和 γ 辐射。

The cyclins are a family of cell cycle-related proteins that are expressed at different times and are involved in regulation of cells through the various phases of the cell cycle. In general, cyclins D and E appear primarily during G1 phase, cyclin A during S and cyclin B in G2 phase. The cyclins complex with cyclin-dependent kinases and these complexes phosphorylate proteins that are important for traverse of cells through the cell cycle. Antibodies are now available to the major cyclins and utilization of these probes for analysis of cyclin expression provides an important tool for measuring the position and frequency of cells in the various phases of the cell cycle. Darzynkiewicz and co-workers have developed and utilized cell-labeling and FCM techniques to demonstrate the correlation of cyclin D and E expression and staurosporine-induced G1 arrest of human lymphocytes and the effects of G2 arrest and polyploidization of transformed cells and cyclin B. U tilizing similar method we are investigating the expression of cyclins on human diploid fibroblasts following exposure to alpha and gamma irradiation.