FACSAria II Cell Sorter

FACSAria II 细胞分选仪

• 批准号: 7791758
• 负责人: ZBIGNIEW DARZYNKIEWICZ
• 金额: $ 50万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-20 至 2011-02-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791758
• 关键词: Area; Cells; Complex; Coronary Vessels; DNA Damage; Diabetes Mellitus; Environment; Heart Diseases; Kidney Diseases; Lyme Disease; Mutagens; New York; Population; Pulmonary Hypertension; Research; Research Personnel; Research Project Grants; Sorting - Cell Movement; Speed; Sterility; United States National Institutes of Health; Work; cell type; instrument; medical schools; particle

DESCRIPTION (provided by applicant): The broad, long term objective of this application is to provide investigators at New York Medical College with a FACSAria II high-speed cell sorter to advance studies in important research areas including diabetes, Lyme disease, renal disease, pulmonary hypertension, coronary vessel and heart disease, and DNA damage caused by genotoxic agents. There are no instruments available in the surrounding area that can provide sterile, high- speed isolation of important cell types or multi-parameter analysis of complex cell populations. The many important research projects currently supported by the National Institutes of Health would benefit tremendously by having access to an instrument that could analyze highly complex populations and identify relatively rare cells that could then be purified and used for further experimentation. The current, outdated Coulter Epics ESP cell sorter cannot provide the speed nor accuracy in sorting required of the various investigators. The new instrument would provide both the biosafety necessary for the operator to work with cells containing infectious particles as well as provide high yields of cells in a sterile environment.

描述（由申请人提供）：本申请的广泛、长期目标是为纽约医学院的研究人员提供FACSAria II高速细胞分选仪，以推进重要研究领域的研究，包括糖尿病、莱姆病、肾脏疾病、肺动脉高压、冠状动脉血管和心脏病以及遗传毒性药物引起的DNA损伤。在周围地区没有仪器可以提供重要细胞类型的无菌、高速分离或复杂细胞群的多参数分析。目前由美国国立卫生研究院支持的许多重要研究项目将受益匪浅，因为他们可以使用一种仪器来分析高度复杂的群体，并识别相对罕见的细胞，然后将其纯化并用于进一步的实验。当前过时的Coulter Epics ESP细胞分选仪无法提供各种研究者所需的分选速度和准确性。新仪器将为操作人员提供处理含有感染性颗粒的细胞所需的生物安全性，并在无菌环境中提供高产量的细胞。