REGULATION OF GENE ACTIVITY

表皮生长因子受体(EGFR)在许多人类肿瘤中过度表达 它在某些癌症的发病机制中起作用。研究表皮生长因子受体的调控 该基因的启动子区域被分离出来，并且含有许多 转录因子结合位点。表皮生长因子受体的表达 (EGFR)基因受富含GC启动子的相互作用控制 激活转录因子基因的区域(SPL和ETF) 或抑制(GCF)转录。来研究EGFR与 表达和GCF的表达，我们分析了GCFRNA的表达水平 人体组织和癌细胞系。我们还检查了 用调节EGFR的药物处理细胞后的GCFRNA 表情。三个RNA物种与GCFcDNA和GCF片段杂交 用cDNA来确定这些RNA之间的关系 物种。GCF基因的染色体定位已被证实 用原位杂交法测定。GCF基因已被放置在 金属诱导金属硫蛋白启动子及三条线的调控 已经产生了表达GCF mRNA的转基因小鼠。

The EGF receptor (EGFR) is overexpressed in many human tumors implicating it in the pathogenesis of some cancers. To study regulation of the EGFR gene, the promoter region was isolated and shown to contain many transcription factor binding sites. The expression of the EGF receptor (EGFR) gene is governed by the interaction with the GC-rich promoter region of the gene of transcription factors that activate (Spl and ETF) or repress (GCF) transcription. To examine the relationship between EGFR expression and GCF expression, we have analyzed the level of GCF RNA in human tissues and cancer cell lines. We have also examined the level of GCF RNA after treatment of cells with agents that regulate EGFR expression. Three RNA species hybridize to the GCF cDNA and fragments of the cDNA have been used to determine the relationship of these RNA species. The chromosomal localization of the GCF gene has been determined by in situ hybridization. The GCF cDNA has been placed under control of the metal inducible metallothionein promoter and three lines of transgenic mice have been generated that express GCF mRNA.