BACTERIAL FUNCTION INVOLVED IN CELL GROWTH CONTROL

我们一直在研究蛋白质降解在 调控基因表达，并启动了一项关于两者之间联系的研究 细胞内染色体合成与染色体分裂之间的关系 组织。我们继续研究生物多样性的调节机制。 胶囊合成，作为了解不稳定作用的模型 蛋白质作为调节器。图中显示了Lon蛋白酶的靶标RCSA 在温度敏感的情况下与另一个调节器RCSB交互 举止。对RCSA表达的研究证实了它的反式作用 在调控RCSA的结构基因下游的一个位置。我们有 一种新的脱脂蛋白水解酶活性的继续研究 Lon底物，RCSA和Sula，这是当一个多拷贝质粒出现时 携带一种我们称之为碱性磷酸酶的基因被引入细胞。新的 活动依赖于一个神秘的前噬菌体的切除以及由此而来的 编码稳定的核糖核酸的染色体基因的失活。三分之一 所研究的能量依赖蛋白水解酶是一种双组分的CLP水解酶。 我们正在继续对CIPP亚单位进行突变研究，以确定 依赖CIPP的CIPP组装加工的特异性和作用 和蛋白水解性。在另一个单独的项目中，抗樟脑 流式细胞术显示了四个不同基因座的突变 导致倍性增加。结合之前的遗传分析， 这表明这些突变(称为MBR)定义了三种不同的 染色体数目和细胞周期的耦合事件。

We have been studying the role that protein degradation plays in regulating gene expression and have initiated a study on the linkages between chromosome synthesis and partition of chromosomes during cell division. We have continued our study of the mechanism of regulation of capsule synthesis, as a model for understanding the role of unstable proteins as regulators. RcsA, a target for the Lon protease, was shown to interact with another regulator, RcsB, in a temperature sensitive manner. Studies on rcsA expression have demonstrated a trans-acting role for a site downstream of the structural gene in regulating rcsA. We have continued studies on a new proteolytic activity capable of degading the Lon substrates, RcsA and SulA, which appears when a multicopy plasmid carrying a gene we have called alp is introduced into cells. The new activity is dependent on excision of a cryptic prophage and consequent inactivation of a chromosomal gene encoding a stable RNA. A third energy-dependent protease under study is the two component Clp protease. We are continuing mutagenesis studies of the CIpP subunit to determine the specificity and role of CIpP-dependent CIpP processing for assembly and proteolytic activity. In a separate project, camphor resistant mutations in four different loci have been shown by flow cytometry to lead to increased ploidy. In combination with previous genetic analysis, this suggests these mutations (called mbr) define three different coupling events for chromosome number and cell cycle.