COAMPLIFICATION OF HIV AND HBV VIRAL DNAS

我们用PCR同时检测HIV 1型肝炎病毒DNA， 使用巢式HBV引物扩增。 扩增产物 使用标记引物检测内部合成区域。 使用 HIV-1和HBV质粒DNA作为对照，我们能够检测到较少的 每个病毒基因组的拷贝数超过10个。 临床血清样本分析 来自双重感染个体的PCR结果均为阳性， HBV和HIV探针，而两种抗体/抗原非反应性标本， PCR阴性。 因此，我们证明了检测多个 在相同的PCR反应中检测临床样品中的病毒种类。 这可能证明 用于分析具有有限体积的样品并简化 侦测

We used PCR simultaneously detect both HIV 1 Hepatitis DNA by co- amplification using nested HBV primers. The amplified products were detected using labeled primers to internally synthesized regions. Using the HIV-1 and HBV plasmid DNAs as controls, we were able to detect less than 10 copies of each viral genome. Analysis of clinical serum samples from dually infected individuals resulted in positive PCR results with both HBV and HIV probes whereas two antibody/antigen non-reactive specimens were also PCR negative. Thus, we demonstrated the ability to detect multiple viral species in clinical samples in the same PCR reaction. This may prove useful for analysis of samples with limited volumes and to simplify detection.