CULTIVATION OF MYCOBACTERIUM LEPRAE

麻风杆菌的体外增殖已经获得，并继续进行了 每隔30-60天进行9次传代。每毫升最好的数字是 令人失望，稳定在每毫升1X10(6)-1X10(7)。可能是因为 需要开发一套连续生长、大容量的发酵罐系统来 获取毫克或克量的湿性或干性生长。生长辅助剂 正被用来试图提高增长率以及 细胞采集。 分枝杆菌的一个新种，一种“犰螂衍生的分枝杆菌”(ADM)， 也已经成长起来。我们可以通过以下方法迅速将其与麻风杆菌区分开来 吡啶脱色，使麻风杆菌不耐酸。 ADM现在已经得到了充分的描述。根据生理学， 从遗传学和抗原性研究来看，它是一个新物种。 麻风分枝杆菌体外培养的最终实验结果 延迟了。卡维尔汉森疾病中心的斯科特·弗兰兹布劳是一位 这项研究的合作者。他一直在为我们提供麻风杆菌细胞 从裸鼠的足垫感染中提纯。他还表演 对该物种进行了决定性的测试。我们可以从麻风杆菌那里获取麻风杆菌材料 受感染的螳螂(肝脏)。犰螂衍生的麻风杆菌总是有一种 一种由螳螂衍生的分支杆菌或ADM混合而成的混合物。大约有二十个 这些物种，还没有被命名。ADM生长过度，M。 在我们的养殖技术中有麻风病。斯科特·弗兰兹布劳的麻风菌感染裸体 鼠群最近在一场流行病中被消灭了。这些老鼠需要一种 一年就会出现感染。我们必须等这么久才能完成我们的 实验。

Multiplication of M. leprae has been obtained in vitro, and continued for nine passages at 30-60 day intervals. Best numbers per milliliter have been disappointing, leveling off at 1X10(6) - 1X10(7) per milliliter. It may be necessary to develop a continuous growth, large volume fermentor system to acquire milligram or gram amounts of wet or dry growth. Growth adjuvants are being used in an attempt to increase the growth rate as well as the cell harvest. A new species of mycobacterium, an "armadillo-derived mycobacterium" (ADM), has also grown out. We can quickly differentiate it from M. leprae by pyridine decolorization, which renders M. leprae, non-acid-fast. The ADM has now been fully characterized. According to physiological, genetic, and antigenic studies, it is a new species. Final experiments on the in vitro culture of Mycobacterium leprae are delayed. Scott Franzblau of the Hansen's disease center at Carville is a collaborator in this study. He has been supplying us with M. leprae cells purified from foot pad infection in the nude mouse. He also performs definitive tests for the species. We have access to M. leprae material from infected armadillos (the liver). Armadillo-derived M. leprae always has an admixture of armadillo-derived mycobacteria, or ADM. There are about twenty species of these, which have not been given names. The ADM overgrow M. leprae in our culture technique. Scott Franzblau's M. leprae infected nude mouse colony was recently wiped out with an epizootic. These mice take a year to develop infections. We must wait this long to finish out our experiments.