BIOLOGY OF HUMAN AIDS RETROVIRUS

该项目的主要目标是开发一个 用于传染性HIV的计数测定。 在初始阶段，我们 在小鼠中产生了一系列单克隆抗体， 识别HIV包膜蛋白。 初步结果 表明这些抗体可以检测到活体上的HIV抗原， 感染的细胞以及甲醇固定的感染的细胞，通过间接 免疫荧光 目前正在进行研究， 确定两种包膜糖蛋白成分中的哪一种 被这些单克隆抗体识别。 随后 抗体将被用来检查是否感染艾滋病毒的细胞 艾滋病患者血液中存在的抗体可以用流式细胞仪检测 分析. 我们还在继续努力开发敏感细胞系 其可用于感染性HIV的测定。 方法 包括将敏感悬浮细胞系与细胞系融合 能够附着在塑料表面上，也能转移CD- 4个基因进入各种附着的细胞系。 这些细胞系现在 他们正在进行体外感染艾滋病病毒的能力的筛选。

The primary goal of this project has been to develop an enumeration assay for infectious HIV. In an initial phase we generated a series of monoclonal antibodies in mice which recognize the HIV envelope protein. The preliminary results indicate that the antibodies will detect HIV antigens on live, infected cells as well as methanol-fixed, infected cells by indirect immunofluorescence. Current studies are in progress to determine which of the two envelope glycoprotein components are recognized by these monoclonal antibodies. Subsequently the antibodies will be used to examine whether HIV infected cells present in blood of AIDS patients can be detected using FACS analysis. We are also continuing our efforts to develop a sensitive cell line which can be used in assays for infectious HIV. Approaches include fusion of sensitive suspension cell lines to cell lines capable of attaching to plastic surfaces as well as transfer of CD- 4 genes into various attached cell lines. These cell lines are now being screened for their ability to be infected in vitro by HIV.