MOLECULAR GENETIC ANALYSIS OF LYMPHOCYTE FUNCTION

该实验室目前的重点是详细了解 细胞表面分子的结构/功能关系 在免疫反应中。特别是，我们已经取得了重大进展 过去一年在几个领域取得的进展：a.爆炸性 用于产生一组细胞系的分子遗传学技术 表达小鼠I类基因的可溶型同源基因 组织相容性抗原H-2Kb、H-2DD、H-2LD和H-2Kk；AS 以及H-2Kb突变体H-2Kbm10；B.使用纯化的可溶性 来自H-2DD和H-2Kb的分子试图生长晶体以 X射线结晶学；C.使用纯化的可溶性分子来 H-2DD的生化和功能行为研究 D.体外表达和功能分析 套索分支点突变有望控制选择 H-2kb和H-2DD第八外显子的剪接受体位点； 通过测试关于耐受性和T细胞教育的假设 产生表达可溶性对应物的转基因小鼠 H-2dd和H-2kb基因。

The ongoing focus of the laboratory is to understand in detail the structure/function relationships of cell surface molecules involved in the immune response. In particular, we have made significant progress over the past year in several areas: A. exploting molecular genetic techniques to generate a panel of cell lines expressing soluble counterparts of the murine class I major histocompatibility antigens H-2Kb, H-2Dd, H-2Ld, and H-2Kk; as well as the H-2Kb mutant H-2Kbm10; B. using the purified soluble molecules from H-2Dd and H-2Kb to attempt to grow crystals for X-ray crystallography; C. using the purified soluble molecules to study the biochemical and functional behavior of the H-2Dd molecule; D. analysis of the expression and function of in vitro lariat branch point mutations expected to control the choice of splice acceptor sites for the eighth exon of H-2Kb and H-2Dd; E. testing hypotheses about tolerance and T cell education by generating transgenic mice that express the soluble counterpart of the H-2Dd and H-2Kb genes.