EFFECTS OF D-LIMONENE ON ALPHA 2U-GLOBULIN IN RAT KIDNEY

d-柠檬烯是多种食品和饮料的天然成分， 在许多水果，蔬菜，肉类，物种和其他食物中发现。 最近NTP发现在大鼠中进行了为期两年的d-柠檬烯慢性研究 和小鼠，并发现有明确的致癌活性的证据， 仅雄性F344大鼠，如肾小管细胞发生率增加所示 肾的增生、腺瘤和腺癌。 响应 在雄性大鼠中观察到的可能与特定的肾脏α干扰有关 2U-球蛋白，雄性大鼠肾脏特有。 本研究旨在 评估透明小滴形成和α的存在 用酶联免疫吸附试验（ELISA）测定2U-球蛋白。 在肾脏匀浆中。 测量等分试样中的总蛋白， Lowry法测定α 2U-球蛋白。 我们已经证实d-柠檬烯 仅在以下患者中产生显著的剂量相关性α 2U-球蛋白增加： 雄性大鼠 这些结果表明，d-柠檬烯相关的肾毒性 在雄性大鼠中，可能与α 2U-球蛋白的催化活性改变有关， 低分子量蛋白质由肝脏在雄激素作用下合成 控制 因此，我们开发了α 2U-球蛋白的ELISA技术， 改进了我们在NTP未来研究项目中使用的程序 和NIEHS。（这些结果发表在1990年的《毒理学家》杂志上。）

d-Limonene is a natural component of a variety of foods and beverages and is found in many fruits, vegetables, meats, species and other food items. Recently NTP found conducted chronic two-year studies of d-Limonene in rats and mice and found there was clear evidence of carcinogenic activity for male F344 rats only as shown by increased incidences of tubular cell hyperplasia, adenomas and adenocarcinomas of the kidney. The response observed in male rats may be linked to specific renal perturbation of alpha 2U-globulin, unique to the male rat kidney. This study was performed to evaluate the hyaline droplet formation and the presence of alpha 2U-globulin was determined by the enzyme linked immunosorbent assay (ELISA) in the kidney homogenates. Total protein were measured in the aliquots for alpha 2U-globulin by the Lowry method. We have confirmed that d-Limonene produced significant dose-related increase of alpha 2U-globulin only in male rats. These results suggest that d-Limonene associated nephrotoxicity in male rats may be related to altered catabolism of alpha 2U-globulin, a low molecular weight protein synthesized by the liver under androgenic control. Thus we developed the ELISA technique for alpha 2U-globulin and refined the procedures for our use in the future research projects at NTP and NIEHS. (These results were published in the Toxicologist, 1990.)