HIGH-SPEED DIFFERENTIAL STOPPED-FLOW CALORIMETER

一台初步的单通道、高速、停流量热计已经研制成功 用于评估混合速度、流速、压降和 热检测灵敏度。该系统使用变速飞轮 和一个电动离合器，将足够的能量传递给 两个驱动注射器将200微升的试剂体积混合在5个 毫秒或更短。驱动注射器的进气管，以及 混合器和检测室保持恒温 在25到50摄氏度之间，超高速热敏电阻(1 Ms)位于 检测室测量反应过程中的温升。一个 使用了新的驱动凸轮，它产生恒定的加速度 注射器。混合室包含一个石英观察管，以允许 反应的光学测量。观察管“漂浮”进来 O形环密封，以减少混合过程中的冲击负荷。的最新更改 仪器包括添加第二个相同的通道，以允许 需要进行差动测量。

A preliminary single-channel, high-speed, stopped-flow calorimeter has been constructed to evaluate mixing speeds, flow velocities, pressure drops, and thermal detection sensitivity. The system uses a variable-speed flywheel and an electrically actuated clutch to transfer sufficient energy to the two drive syringes to mix reagent volumes of 200 microliters in 5 milliseconds or less. The inlet tubes from the drive syringes, as well as the mixer and the detection chamber, are kept at constant temperature between 25 and 50 deg C. An ultra-fast thermistor (1 ms) is located in the detection chamber to measure the temperature rise during the reaction. A new drive cam is used, which produces a constant acceleration of the syringes. The mixing chamber contains a quartz observation tube to allow optical measurement of the reaction. The observation tube 'floats' in O-ring seals to reduce shock loads during mixing. The latest change to the instrument involved adding a second, identical channel to allow differential measurements to be made.