HIGH SPEED DIFFERENTIAL STOPPED FLOW CALORIMETER

一种初步的单通道、高速、停流量热计已经研制成功 用于评估混合速度、流速、压降和 热检测灵敏度。该系统使用变速飞轮 和电动离合器，将足够的能量传递给这两个 驱动注射器在5毫秒内混合200微升的试剂 或者更少。驱动注射器的进气管，以及混合器和 将检测室保持在25至50之间恒温 检测室内装有超快热敏电阻(Ims)。 来测量反应过程中的温度升高。一种新的传动凸轮是 用于产生注射器的持续加速。搅拌机 室内包含一个石英观察管，以允许光学测量 这种反应。观察管漂浮在O形密封圈中以减少冲击 混合过程中的负载。

A preliminary single channel, high speed, stopped-flow calorimeter has been constructed to evaluate mixing speeds, flow velocities, pressure drops, and thermal detection sensitivity. The system uses a variable speed flywheel and electrically actuated clutch to transfer sufficient energy to the two drive syringes to mix reagent volumes of 200 microliters in 5 milliseconds or less. The inlet tubes from the drive syringes, as well as the mixer and the detection chamber are kept at constant temperature between 25 and 50 deg C. An ultra-fast thermistor (I ms) is located in the detection chamber to measure the temperature rise during the reaction. A new drive cam is used which produces a constant acceleration of the syringes. The mixing chamber contains a quartz observation tube to allow optical measurement of the reaction. The observation tube 'floats' in o-ring seals to reduce shock loads during mixing.