A MICROCOMPUTER-BASED FLOW CYTOMETRY DATA COLLECTION AND ANALYSIS SYSTEM

早些时候我们开发了一个电子接口系统，它连接了模拟 从流式细胞仪的四个前置放大器到 微型计算机。该系统收集、处理和调节 由微型计算机采集之前的模拟信号，并且有效 解决了异步和同步数据的接口问题 转换、获取和存储。我们发现当模拟信号 与积分器的重置同时发生，仅部分 获得了积分，这扭曲了细胞直方图。触发器 模拟探测器部分的延时电路消除了这个问题。 在此期间，我们研究了各种接口的时间安排和 详细的计算机任务，以确定数据吞吐量的哪些方面有所提高 都是可能的。

Earlier we developed an electronic interface system which linked the analog signals from the four preamplifiers of the flow cytometer to the microcomputer. This system collected, processed, and conditioned the analog signals before acquisition by the microcomputer, and effectively solved the problem of interfacing asynchronous and synchronous data conversion, acquisition, and storage. We found that when analog signals occurred simultaneously with the reset of the integrators, only partial integrations were obtained, which skewed the cell histogram. A trigger hold-off circuit in the analog detector section eliminated this problem. During this period, we have studied the timing of the various interface and computer tasks in detail to identify where improvements in data throughput were possible.