DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

该计划的目标是促进NIH动物的管理 遗传资源(NIHAGR)的开发利用 胚胎采集、培养、冷冻和移植等技术 和配子，用于冷冻保存遗传物质和提高生殖能力 潜力。NIHAGR维护着一些关键物种，在 物种，生物医学中使用的大量宝贵的基因类型 研究。胚胎保存在冷冻介质中使用冷冻保护剂 它允许足够的脱水来保护卵裂球 冷得要命。在冷冻保护剂解冻和稀释后，胚胎 进行培养以评估体外发育。胚胎移植 受精卵受精和卵子冷冻的受精卵监测器 作为挽救有限遗传物质的技术而被研究 单雌或体外受精率低的品系。存入银行 胚胎被保持在冷冻状态，直到需要重新衍生。一个 实验室工作的主要重点是研究和优化 在小鼠和兔子以及最近在大鼠身上使用的低温银行技术。一个 主要的研究发现是胚胎的冷冻能力被混淆了 受小鼠基因影响显著。这样的回应已经被研究过了 在超过56种基因型中，大多数表现出特定于基因型的存活率 标准化胚胎冷冻-解冻程序的比率。附属项目 是针对改善长期胚胎的比较方面的 储存，特别强调研究低温保护剂和 加强胚胎冷冻、解冻、稀释和移植的方法。 兔子的工作重点是改进收集程序和 胚胎的保存。

The program objective is to facilitate the management of the NIH animal Genetic Resource (NIHAGR) through the development and utilization of techniques such as collection, culture, freezing and transfer of embryos and gametes for cryobanking genetic material and improving reproductive potential. The NIHAGR maintains a number of critical species and, within species , a vast number of invaluable genotypes used in biomedical research. Embryo preservation uses a cryoprotectant in the freezing medium which allows sufficient dehydration to protect the blastomeres during freezing. Following thawing and dilution of the cryoprotectant, embryos are cultured to assess in vitro development. Transplantation of embryos into recipient females monitors in vitro fertilization and ova freezing are being examined as techniques to salvage limited genetic material from single females or strains with poor in vitro fertilization rates. Banked embryos are maintained in a frozen state until needed for rederivation. A primary focus of laboratory efforts is the study and optimization of cryobanking technology in mice and rabbits and more recently, in rats. A major research finding is that embryo freezability is confounded and influenced markedly by mouse genotype. Such responses have been examined in over 56 genotypes, most of which display genotype-specific survival rates to standardize embryo freeze-thawing procedures. Ancillary projects are oriented toward the comparative aspects of improving long-term embryo storage with particular emphasis on the study of cryoprotectants and enhancing methods for embryo freezing, thawing, dilution and transfer. Efforts in the rabbit focus on improvements in collection procedures and preservation of embryos.