EFFECTS OF D-LIMONENE ON ALPHA 2U-GLOBULIN IN RAT KIDNEY

D-柠檬烯是多种食物的天然成分， 存在于许多水果、蔬菜、肉类、香料中 和其他食物。最近，NTP进行了为期两年的慢性 D-柠檬烯在大鼠和小鼠体内的研究发现 仅对雄性F344大鼠具有致癌活性的证据如下所示 肾小管上皮细胞不典型增生、腺瘤和 肾腺癌。雄性大鼠的反应观察 可能与α2U-g1obin的特异性肾脏扰动有关， 这是雄性大鼠肾脏独有的。这项研究是为了 评估透明液滴的形成和阿尔法的存在 雄性和雌性F344大鼠14岁后肾脏中的U-球蛋白 按0、75、150、300、600或1200剂量灌胃的天数 玉米油中的d-柠檬烯为mg/kg。测定α2U球蛋白 用酶联免疫吸附试验(ELISA法)测定肾脏 匀浆。总蛋白在相同等分中被测量 Lowry法测得α2U-g1obu1 in。我们已经确认了d- 柠檬烯能显著增加α2U- 球蛋白仅存在于雄性大鼠体内。这些结果表明，d-柠檬烯 雄性大鼠的相关肾毒性可能与肾毒性改变有关 低分子蛋白质α2U球蛋白的分解代谢 由肝脏在雄激素控制下合成。

d-Limonene is a natural component of a variety of foods and beverages and is found in many fruits, vegetables, meats, spices and other food items. Recently NTP conducted chronic two-year studies of d-Limonene in rats and mice and found there was clear evidence of carcinogenic activity for male F344 rats only as shown by increased incidences of tubular cell nyperplasia, adenomas and adenocarcinomas of the kidney. The response observed in male rats may be linked to specific renal pertubation of alpha 2U-g1obulin, unique to the male rat kidney. This study was performed to evaluate the hyaline droplet formation and the presence of alpha 2U-globulin in the kidney of male and female F344 rats after 14 days of gavage dosing at doses of 0, 75, 150, 300, 600 or 1200 mg/kg d-Limonene in corn oil. The alpha 2U-globulin was determined by the enzyme linked immunosoroent assay (ELISA) in the kidney homogenates. Total protein were measured in the same aliquots for alpha 2U-g1obu1in by the Lowry method. We have confirmed that d- Limonene produced significant dose-related increase of alpha 2U- globulin only in male rats. These results suggest that d-Limonene associated nephrotoxicity in male rats may be related to altered catabolism of alpha 2U-globulin, a low molecular weight protein synthesized by the liver under androgenic control.