INTERACTION BETWEEN CEREBRAL CAPILLARY ENDOTHELIUM AND IMMUNE CELLS

这项研究代表了一项不断努力的发展和建立 最可靠、最具重复性的培养方法 易感SJL小鼠脑微血管内皮细胞来源 致EAE。几种不同的隔离、解离程序 并对细胞的播种进行评估，以获得最大 内皮细胞的产量。它们的生长和繁殖 在内皮细胞存在或不存在的情况下确定细胞 生长因子(ECGF)。 这些调查虽然不完整，但表明小鼠 内皮细胞需要纤维连接蛋白基质和ECGF 添加的培养基对试管苗的生长和繁殖效果最佳。 每个测试过程与细胞产量无关 不干扰因子VIII的表达。诱导 I-A+抗原与血管内皮细胞增殖反应 在所有血管内皮细胞培养中都可以使用干扰素-γ。因此， 已建立的小鼠EC培养适合于设计模型 研究脑内皮细胞和脑内皮细胞之间相互作用的系统 处于生存状态的免疫细胞，这在 对几种神经系统疾病发病机制的认识 精神错乱。

This study represents a continuous effort to develop and establish the most reliable and reproducible method for culturing cerebromicrovascular endothelium derived from SJL mice susceptible to EAE. Several different procedures of isolation, dissociation and seeding of the cells were evaluated to obtain the greatest yield of the endothelium. The growth and propagation of these cells was determined in the presence or absence of endothelial cell growth factor (ECGF). These investigations, although incomplete indicate that the murine endothelial cells require a fibronectin matrix and ECGF supplemented medium for the best growth and propagation in vitro. Each of the tested procedures irrespective o the cellular yield does not interfere with the expression of Factor VIII. Induction of I-A+ antigen and proliferating response of the endothelium to IFNgamma is possible in all endothelial cultures. Thus the established murine EC cultures are suitable for designing a model system to investigate the interaction between the cerebral EC and immune cells in living state, which are of importance in the understanding of the pathogenesis of several neurological disorders.