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STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT

STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
免疫球蛋白基因重排的研究
批准号：
3940666
负责人：
M GELLERT
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3940666
关键词：
B lymphocyte antibody formation bacterial DNA bioassay gene expression genetic manipulation genetic recombination immunoglobulins molecular biology nucleic acid sequence plasmids tissue /cell culture

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中文摘要
翻译
儿童免疫球蛋白基因V-J重组活性水平的研究 来自淋巴系或非淋巴系的细胞系 检查过了。该检测使用染色体外DNA底物， 并因此避免了与使用 染色体上整合的DNA。重组活动是 发现在B淋巴样细胞发育过程中呈进行性变化。它是 在非常早期阶段低(PRO-GMB)，在PRO-B细胞中高得多， 但随后在后期下降，达到了无法检测到的 在成熟B细胞中的水平。该活动还以多种形式出现 髓系细胞的潜在祖细胞，在前T细胞中但不是 成熟的T细胞。在几种情况下没有发现活性 非造血细胞系。
英文摘要
The level of immunoglobulin gene V-J recombination activity in cell lines derived from lymphoid or nonlymphoid lineages was examined. The assay uses an extrachromosomal DNA substrate, and thus avoids difficulties associated with the use of chromosomally integrated DNA. The recombination activity is found to vary progressively during B lymphoid development. It is low at a very early stage (pro-GMB), much higher in pro-B cells, but then declines through later stages, reaching an undetectable level in mature B cells. The activity is also present in multi potential progenitors of myeloid cells, and in pre-T but not mature T cells. No activity was found in several nonhematopoietic cell lines.
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EFFECTS OF DNA SUPERCOILING ON THE TOPOLOGICAL PROPERTIES OF NUCLEOSOMES
STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
EFFECTS OF DNA SUPERCOILING ON THE TOPOLOGICAL PROPERTIES OF NUCLEOSOMES
EFFECTS OF DNA SUPERCOILING ON THE TOPOLOGICAL PROPERTIES OF NUCLEOSOMES
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