BIOLOGY OF HUMAN AIDS RETROVIRUS

正在努力开发一种传染性艾滋病的计数测定法 逆转录病毒 这样的分析将有助于更准确和灵敏 滴定组织和体液中的病毒，并提供更多 检测抗体中和病毒的灵敏手段。 测定 我们正在开发的技术将涉及体外检测感染灶， 通过致细胞病变效应或局灶性间接 使用病毒特异性抗血清的免疫荧光。 到目前为止， 诱变一个允许艾滋病逆转录病毒的非贴壁细胞系 并衍生出该细胞系的耐药和敏感克隆。 这些克隆已经融合到附着的细胞系上，融合的后代具有 被选中的附着塑料和耐药性。 我们现在 测试融合克隆对艾滋病逆转录病毒感染的敏感性。 这个项目也正在扩大，试图产生艾滋病 可检测细胞表面病毒的病毒特异性单克隆抗体 包膜蛋白并因此在局部免疫荧光中具有潜在用途 艾滋病病毒检测。

Effort is underway to develop an enumeration assay for infectious AIDS retrovirus. Such an assay would facilitate more accurate and sensitive titration of virus in tissues and body fluids as well as provide a more sensitive means of detecting virus neutralization by antibodies. The assay we are developing would involve detection of foci of infection in vitro in attached cell monolayers by cytopathic effect or focal indirect immunofluorescence using virus-specific antisera. Thus far, we have mutagenized a nonattached cell line which is permissive for AIDS retrovirus and have derived drug resistant and sensitive clones of this cell line. These clones have been fused to attached cell lines and fused progeny have been selected for attachment to plastic and drug resistance. We are now testing fused clones for susceptibility to infection by AIDS retrovirus. This project is also being expanded to attempt to generate AIDS virus-specific monoclonal antibodies which will detect cell surface virus envelope protein and thus be of potential use in a focal immunofluorescence assay for AIDS virus.