DEVELOPMENT OF A PULSED LIGHT SOURCE, SINGLE PHOTON

检测微量蛋白质的能力在以下两个方面都很有趣 蛋白质化学和免疫学。一种可能的放射免疫替代品 放射免疫分析(RIA)是用笼中的铕分子标记蛋白质。 通过利用Eu(500)很长的荧光寿命 微秒)极高的灵敏度是可能的。脉冲氮气激光器 工作在337 nm，15pps，功率输出200微焦耳 与PET光电倍增管上的1/2英寸端一起使用，以检查简单的 铕标记系统。检测到10-14摩尔的Eu 在100微升的样品中实现。将这一数字扩展到1000万到1600万的努力 目前正在追随着极大改进的技术 光电管、计数电子器件和激光器的可靠性 光源。这种系统的可能用途之一是在 检测到极低水平的逆转录病毒。

The ability to detect minute amounts of protein is of interest both in protein chemistry and immunology. A possible replacement for radioimmuno assay (RIA) is the tagging of the protein by a caged molecule of Europium. By taking advantage of the very long fluorescence lifetime of Europium (500 microseconds) extreme sensitivities are possible. A pulsed nitrogen laser operating at 337 nm at 15 pps and power output of 200 microjoules has been used with a 1/2 inch end on PET photomultiplier tube to examine a simple Europium tagged system. Detection of 10-14 molar Europium was readily achieved in a 100 microliter sample. Efforts to extend this to 10-16 M are presently being pursued along with techniques for greatly improving reliability of both the phototube, counting electronics, and the laser light source. One of the possible uses of such a system is in the detection of very low levels of retrovirus.