DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

该计划的目标是促进NIH动物的管理 胚胎发育利用中的遗传资源 采集、培养、冷冻和移植技术 对遗传物质进行超低温储存，提高生殖潜力。这个 NIHAGR由几个实验室物种组成，对这些物种来说，低温储存是 在小鼠身上进行，在兔子身上发展。冷藏库被搞糊涂了 通过发现30多只小鼠对冷冻保存的独特基因反应 到目前为止所调查的基因类型。胚胎被冷冻在一个可编程的单元中 以物种特定的比率确保胚胎存活。 冷冻介质中的冷冻保护剂允许充分脱水以 在冷冻期间保护卵裂球。在解冻和稀释后 冷冻保护剂胚胎被培养以评估体外发育。 将胚胎移植到受体雌性体内的监视器 发展。体外受精和卵子冷冻正在被检测为 从单身女性身上抢救有限遗传物质的技术 体内受精率低的菌株。储存的胚胎是 保持冻结状态，直到需要重新派生。并发 比较研究的目的是确定在 提高女性对诱导排卵反应的方法学 方案，提高解冻后胚胎的存活率，并检查 基因对胚胎冷冻保存的影响。

The program objective is to facilitate the management of the NIH Animal Genetic Resource (NIHAGR) through development and utilizaton of embryo collection, culture, freezing and transplantation as techniques for cryobanking genetic material and improving reproductive potential. The NIHAGR consists of several laboratory species for which cryobanking is ongoing in mice and being developed in rabbits. Cryobanking is confounded by unique genotypic responses to cryopreservation found in over 30 mouse genotypes investigated to date. Embryos are frozen in a programable unit at species specific rates to ensure embryo survival. A cryoprotectant in the freezing medium allows sufficient dehydration to protect the blastomeres during freezing. Following thawing and dilution of the cryoprotectant, embryos are cultured to assess in vitro development. Transplantation of embryos into recipient females monitors in vivo development. In vitro fertilization and ova freezing are being examined as tehcniques to salvage limited genetic material from single females or strains with poor in vivo fertilizatiaon rates. Banked embryos are maintained in a frozen state until needed for rederivation. Concurrent comparative research studies are designed to identify modifications in methodology which will enhance female response to ovulation induction regimens, improve post-thaw embryo survival and examine the influence of genotype on embryo cryopreservation.